Influence of insertion sequences on population structure of phytopathogenic bacteria in the Ralstonia solanacearum species complex.
Influence of insertion sequences on population structure of phytopathogenic bacteria in the Ralstonia solanacearum species complex.
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DOI：
10.1099/mic.0.001364
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发表时间：
2023-07
期刊：
影响因子：
2.8
通讯作者：
Friman, Ville-Petri
中科院分区：
文献类型：
--
作者：
Greenrod, Samuel T. E.;Stoycheva, Martina;Elphinstone, John;Friman, Ville-Petri
关键词：
青枯菌 种复合体（RSSC）是植物病原细菌的一个破坏性类群，是引起植物青枯病的病原菌。实验研究将RSSC的毒力归因于插入序列（IS），即可以破坏和激活宿主基因的转座遗传元件。然而，RSSC IS的全球多样性和分布是未知的。在这项研究中，IS的生物信息学确定的356 RSSC菌株代表5个系统发育谱系和遗传距离的措施和比较的基础上与ISONS数据库的多样性进行了研究的多样化集合。IS系统发生关联是基于它们在RSSC系统发生中的分布来确定的。此外，基因组内的IS位置进行了表征，并根据IS与编码序列的接近程度确定其潜在的基因破坏。总共，我们发现24732 IS属于11个IS家族和26个IS亚组，其中超过一半的IS存在于巨质粒中。虽然IS家族普遍分布于RSSC系统发生中，但IS亚组显示出强烈的谱系特异性分布，并且遗传相似的细菌分离株具有相似的IS含量。与细菌宿主遗传背景的类似关联也被观察到与IS插入位置，其在密切相关的细菌分离株中高度保守。最后，IS被发现破坏了在毒力，胁迫耐受性和代谢方面具有预测功能的基因，这表明它们可能是适应性的。这项研究强调，RSSC插入序列跟踪其细菌宿主的进化，可能有助于内部和谱系间的遗传多样性。
Ralstonia solanacearum species complex (RSSC) is a destructive group of plant pathogenic bacteria and the causative agent of bacterial wilt disease. Experimental studies have attributed RSSC virulence to insertion sequences (IS), transposable genetic elements which can both disrupt and activate host genes. Yet, the global diversity and distribution of RSSC IS are unknown. In this study, IS were bioinformatically identified in a diverse collection of 356 RSSC isolates representing five phylogenetic lineages and their diversity investigated based on genetic distance measures and comparisons with the ISFinder database. IS phylogenetic associations were determined based on their distribution across the RSSC phylogeny. Moreover, IS positions within genomes were characterised and their potential gene disruptions determined based on IS proximity to coding sequences. In total, we found 24732 IS belonging to eleven IS families and 26 IS subgroups with over half of the IS found in the megaplasmid. While IS families were generally widespread across the RSSC phylogeny, IS subgroups showed strong lineage-specific distributions and genetically similar bacterial isolates had similar IS contents. Similar associations with bacterial host genetic background were also observed with IS insertion positions which were highly conserved in closely related bacterial isolates. Finally, IS were found to disrupt genes with predicted functions in virulence, stress tolerance, and metabolism suggesting that they might be adaptive. This study highlights that RSSC insertion sequences track the evolution of their bacterial hosts potentially contributing to both intra- and inter-lineage genetic diversity.
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影响因子：
2.1
作者：
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