Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture.
Rat liver ECM incorporated into electrospun polycaprolactone scaffolds as a platform for hepatocyte culture.
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DOI：
10.1002/jbm.b.35115
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发表时间：
2022-12
影响因子：
3.4
通讯作者：
Callanan, Anthony
中科院分区：
文献类型：
--
作者：
Bate, Thomas S. R.;Shanahan, William;Casillo, Joseph P.;Grant, Rhiannon;Forbes, Stuart J.;Callanan, Anthony
肝病正在全球蔓延；然而，卫生保健系统仍然缺乏经批准的药物治疗策略来减轻潜在的肝衰竭。器官移植是治疗肝功能衰竭的唯一方法，随着肝脏疾病病例的增加，移植计划越来越不能为所有患者提供及时的移植。制定药物缓解战略显然是必要的，改进药物开发过程的方法被认为是为此目的至关重要的。在此，我们提出了一种将全器官脱细胞大鼠肝脏ECM （rLECM）纳入聚己内酯（PCL）电纺丝支架的方法，目的是生产生物学相关的肝组织模型。用5 w/w%和10 w/w%的rLECM:PCL制备了rLECM:PCL支架，并对其进行了SEM成像、拉伸力学分析和FTIR光谱分析。将肝癌细胞株HepG2在支架上培养14天，通过细胞活力测定、DNA定量、白蛋白定量、免疫组织化学和RT - qPCR基因表达分析进行分析。结果显示，HepG2在含有rLECM的支架上的增殖活性显著增加，同时关键基因的表达保持不变。本研究证实，rLECM可用于调节电纺丝PCL支架的生物活性，并有可能生产出适合肝细胞培养和体外肝组织模型的电纺丝支架。
Liver disease is expanding across the globe; however, health‐care systems still lack approved pharmaceutical treatment strategies to mitigate potential liver failures. Organ transplantation is the only treatment for liver failure and with increasing cases of liver disease, transplant programs increasingly cannot provide timely transplant availability for all patients. The development of pharmaceutical mitigation strategies is clearly necessary and methods to improve drug development processes are considered vital for this purpose. Herein, we present a methodology for incorporating whole organ decellularised rat liver ECM (rLECM) into polycaprolactone (PCL) electrospun scaffolds with the aim of producing biologically relevant liver tissue models. rLECM PCL scaffolds have been produced with 5 w/w% and 10 w/w% rLECM:PCL and were analyzed by SEM imaging, tensile mechanical analyses and FTIR spectroscopy. The hepatocellular carcinoma cell line, HepG2, was cultured upon the scaffolds for 14 days and were analyzed through cell viability assay, DNA quantification, albumin quantification, immunohistochemistry, and RT‐qPCR gene expression analysis. Results showed significant increases in proliferative activity of HepG2 on rLECM containing scaffolds alongside maintained key gene expression. This study confirms that rLECM can be utilized to modulate the bioactivity of electrospun PCL scaffolds and has the potential to produce electrospun scaffolds suitable for enhanced hepatocyte cultures and in‐vitro liver tissue models.
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HEPG2和原代小鼠肝细胞对电纺PCL支架的形态变化的反应差异。
影响因子：
4.6
作者：
Bate TSR;Gadd VL;Forbes SJ;Callanan A
通讯作者：
Callanan A
DOI：
10.1002/jbm.b.33668
发表时间：
2017-08-01
影响因子：
3.4
作者：
He, M.;Callanan, A.;Stevens, M. M.
通讯作者：
Stevens, M. M.
影响因子：
4.3
作者：
Cozzolino AM;Noce V;Battistelli C;Marchetti A;Grassi G;Cicchini C;Tripodi M;Amicone L
通讯作者：
Amicone L
影响因子：
25.7
作者：
Asrani, Sumeet K.;Devarbhavi, Harshad;Kamath, Patrick S.
通讯作者：
Kamath, Patrick S.
细胞外基质组合物的改变指导受损的肝组织的修复。
影响因子：
4.6
作者：
Klaas M;Kangur T;Viil J;Mäemets-Allas K;Minajeva A;Vadi K;Antsov M;Lapidus N;Järvekülg M;Jaks V
通讯作者：
Jaks V