Syndecan-4 and stromal cell-derived factor-1 alpha functionalized endovascular scaffold facilitates adhesion, spreading and differentiation of endothelial colony forming cells and functions under flow and shear stress conditions.
Syndecan-4 and stromal cell-derived factor-1 alpha functionalized endovascular scaffold facilitates adhesion, spreading and differentiation of endothelial colony forming cells and functions under flow and shear stress conditions.
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DOI：
10.1002/jbm.b.35170
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发表时间：
2023-03
影响因子：
3.4
通讯作者：
Wagner, William D.
中科院分区：
文献类型：
--
作者：
Wu, Yidi;Yazdani, Saami K.;Bolander, Johanna Elin Marie;Wagner, William D.
具有捕获分子的脱细胞血管支架在招募循环内皮集落形成细胞（ecfc）以促进体内内皮化方面显示出巨大的前景。初始细胞捕获后有利于细胞扩散和分化的微环境是最终形成功能性内皮的关键。在这项研究中，syndecan - 4和基质细胞衍生因子- 1 α被用来功能化一种由聚甘油脂酸、丝素蛋白和I型胶原组成的弹性生物材料，称为PFC，以增强ECFC -材料的相互作用。功能化PFC （fPFC）在生理流量下表现出更强的ecfc捕获能力。早在2 h， fPFC上的单个细胞扩散面积（1474±63 μm2）就显著大于PFC(1187±54 μm2)，表明细胞-物质相互作用增强。此外，与组织培养塑料相比，7天后fPFC显著上调了内皮细胞特异性标志物的表达，如血小板内皮细胞粘附分子（24倍）和血管性血血病因子（11倍），表明ecfc向内皮细胞分化。fPFC制备成小直径导管，并在脉动血流量生物反应器上进行了测试，结果表明其性能稳定。研究结果表明，本文提出的新的表面功能化策略导致血管内材料具有增强的内皮化。
Acellular vascular scaffolds with capture molecules have shown great promise in recruiting circulating endothelial colony forming cells (ECFCs) to promote in vivo endothelialization. A microenvironment conducive to cell spreading and differentiation following initial cell capture are key to the eventual formation of a functional endothelium. In this study, syndecan‐4 and stromal cell‐derived factor‐1 alpha were used to functionalize an elastomeric biomaterial composed of poly(glycerol sebacate), Silk Fibroin and Type I Collagen, termed PFC, to enhance ECFC‐material interaction. Functionalized PFC (fPFC) showed significantly greater ECFCs capture capability under physiological flow. Individual cell spreading area on fPFC (1474 ± 63 μm2) was significantly greater than on PFC (1187 ± 54 μm2) as early as 2 h, indicating enhanced cell–material interaction. Moreover, fPFC significantly upregulated the expression of endothelial cell specific markers such as platelet endothelial cell adhesion molecule (24‐fold) and Von Willebrand Factor (11‐fold) compared with tissue culture plastic after 7 days, demonstrating differentiation of ECFCs into endothelial cells. fPFC fabricated as small diameter conduits and tested using a pulsatile blood flow bioreactor were stable and maintained function. The findings suggest that the new surface functionalization strategy proposed here results in an endovascular material with enhanced endothelialization.
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影响因子：
2.9
作者：
Billings PC;Pacifici M
通讯作者：
Pacifici M
影响因子：
3.1
作者：
Boland, ED;Matthews, JA;Bowlin, GL
通讯作者：
Bowlin, GL
影响因子：
4.6
作者：
Hartman EMJ;De Nisco G;Gijsen FJH;Korteland SA;van der Steen AFW;Daemen J;Wentzel JJ
通讯作者：
Wentzel JJ
将肝素、SDF-1 α 和 CD47 无接头共价固定在 PTFE 表面上，以实现抗血栓形成、内皮化和抗炎
影响因子：
14
作者：
Gao, Ang;Hang, Ruiqiang;Chu, Paul K.
通讯作者：
Chu, Paul K.
影响因子：
19.6
作者：
Ballermann, BJ;Dardik, A;Liu, AL
通讯作者：
Liu, AL