Helicoverpa armigera miR-2055 regulates lipid metabolism via fatty acid synthase expression.
Helicoverpa armigera miR-2055 regulates lipid metabolism via fatty acid synthase expression.
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棉铃虫 miR-2055 通过脂肪酸合酶表达调节脂质代谢
DOI：
10.1098/rsob.210307
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发表时间：
2022-03
期刊：
影响因子：
5.8
通讯作者：
Zou Z
中科院分区：
文献类型：
--
作者：
Cheng Y;Lu T;Guo J;Lin Z;Jin Q;Zhang X;Zou Z
昆虫激素和microRNA调节脂质代谢，但其机制尚未完全阐明。在这里，我们发现，棉铃虫幼虫取食拟南芥（AT）叶有一个较低的三酰甘油（TAG）水平和更延迟的发展比个人喂养人工饲料（AD）。小RNA和mRNA的关联分析显示，与脂质代谢相关的microRNA miR-2055的水平在饲喂AT的幼虫中显著较高。双荧光素酶报告基因检测显示miR-2055与脂肪酸合成酶（FAS）mRNA的3′ UTR结合，抑制FAS的表达。通过注射agomir提高miR-2055在幼虫中的水平降低FAS mRNA和蛋白水平，从而导致脂肪体中游离脂肪酸（FFA）和TAG的减少。有趣的是，体外试验表明，保幼激素（JH）以剂量依赖性方式增加miR-2055的积累，而敲低耐甲氧普烯（Met）或Kruppel同源物1（Kr-h1）则降低miR-2055水平。这意味着JH通过Met-Kr-h1信号诱导miR-2055的表达。这些研究结果表明，JH和miRNA协同调控脂质合成，为昆虫代谢调控机制提供了新的思路。
Insect hormones and microRNAs regulate lipid metabolism, but the mechanisms are not fully elucidated. Here, we found that cotton bollworm larvae feeding on Arabidopsis thaliana (AT) leaves had a lower triacylglycerol (TAG) level and more delayed development than individuals feeding on artificial diet (AD). Association analysis of small RNA and mRNA revealed that the level of miR-2055, a microRNA related to lipid metabolism, was significantly higher in larvae feeding on AT. Dual-luciferase reporter assays demonstrated miR-2055 binding to 3′ UTR of fatty acid synthase (FAS) mRNA to suppress its expression. Elevating the level of miR-2055 in larvae by agomir injection decreased FAS mRNA and protein levels, which resulted in reduction of free fatty acid (FFA) and TAG in fat body. Interestingly, in vitro assays illustrated that juvenile hormone (JH) increased miR-2055 accumulation in a dosage-dependent manner, whereas knockdown of Methoprene tolerant (Met) or Kruppel homologue 1 (Kr-h1) decreased the miR-2055 level. This implied that JH induces the expression of miR-2055 via a Met-Kr-h1 signal. These findings demonstrate that JH and miRNA cooperate to modulate lipid synthesis, which provides new insights into the regulatory mechanisms of metabolism in insects.
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