Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging.
Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging.
复制标题
DOI：
10.1371/journal.pone.0006592
复制
发表时间：
2009-08-11
期刊：
影响因子：
3.7
通讯作者：
Rocke TE
中科院分区：
文献类型：
--
作者：
Osorio JE;Iams KP;Meteyer CU;Rocke TE
猴痘病毒（MPXV）引起人类猴痘，这是一种非洲流行的人畜共患天花样疾病，引起了全世界公共卫生和生物防御的关注。使用来自刚果（MPXV-2003-Congo-358）和西非（MPXV-2003-USA-044）分支的病毒，我们构建了表达荧光素酶基因的重组病毒（MPXV-Congo/Luc+和MPXV-USA-Luc+），并通过生物光子成像比较了它们对小鼠的病毒感染。 BALB/c 小鼠被两个 MPXV 分支感染，但它们在感染后 10 天内恢复并清除了感染 (PI)。然而，严重联合免疫缺陷 (SCID) BALB/c 小鼠的感染导致 100% 致死率。 MPXV-Congo 和 MPXV-Congo/Luc+ 的腹膜内 (IP) 注射均导致全身性临床疾病，且感染后 9 (±0) 天的平均死亡时间相同。同样，与刚果毒株相比，用 MPXV-USA 或 MPXV-USA-Luc+ 腹膜内注射 SCID-BALB/c 小鼠会导致类似的疾病，但两种病毒的平均死亡时间（11±0 天）更长（P<0.05）。 SCID 小鼠的成像研究显示，感染后 24 小时内腹部出现发光，随后扩散到其他地方。感染 MPXV-USA/Luc+ 的动物组织中的发光强度比接种 MPXV-Congo/Luc+ 的动物要弱，并且 MPXV-USA/Luc+ 病毒的全身性传播比 MPXV-Congo/Luc+ 晚约两天。高病毒滴度和免疫组织化学证明，卵巢是病毒复制的重要靶点。这些研究证明了小鼠模型和生物光子成像比较 MPX 病毒的疾病进展和组织向性的适用性。
Monkeypox viruses (MPXV) cause human monkeypox, a zoonotic smallpox-like disease endemic to Africa, and are of worldwide public health and biodefense concern. Using viruses from the Congo (MPXV-2003-Congo-358) and West African (MPXV-2003-USA-044) clades, we constructed recombinant viruses that express the luciferase gene (MPXV-Congo/Luc+and MPXV-USA-Luc+) and compared their viral infection in mice by biophotonic imaging. BALB/c mice became infected by both MPXV clades, but they recovered and cleared the infection within 10 days post-infection (PI). However, infection in severe combined immune deficient (SCID) BALB/c mice resulted in 100% lethality. Intraperitoneal (IP) injection of both MPXV-Congo and MPXV-Congo/Luc+resulted in a systemic clinical disease and the same mean time-to-death at 9 (±0) days post-infection. Likewise, IP injection of SCID-BALB/c mice with MPXV-USA or the MPXV-USA-Luc+, resulted in similar disease but longer (P<0.05) mean time-to-death (11±0 days) for both viruses compared to the Congo strains. Imaging studies in SCID mice showed luminescence in the abdomen within 24 hours PI with subsequent spread elsewhere. Animals infected with the MPXV-USA/Luc+had less intense luminescence in tissues than those inoculated with MPXV-Congo/Luc+, and systemic spread of the MPXV-USA/Luc+virus occurred approximately two days later than the MPXV-Congo/Luc+. The ovary was an important target for viral replication as evidenced by the high viral titers and immunohistochemistry. These studies demonstrate the suitability of a mouse model and biophotonic imaging to compare the disease progression and tissue tropism of MPX viruses.
登录
查看更多内容
影响因子：
9.4
作者：
Meyer, H;Perrichot, M;Formenty, P
通讯作者：
Formenty, P
影响因子：
5.5
作者：
Osorio, JE;Powell, TD;Stinchcomb, DT
通讯作者：
Stinchcomb, DT
影响因子：
5.4
作者：
Luker, GD;Prior, JL;Leib, DA
通讯作者：
Leib, DA
影响因子：
6.4
作者：
Edghill-Smith, Y;Bray, M;Franchini, G
通讯作者：
Franchini, G
Cidofovir治疗痘病毒感染。
影响因子：
7.6
作者：
De Clercq, E
通讯作者：
De Clercq, E