Molecular detection of a novel perkinsid associated with the deep‐sea clam Phreagena okutanii

Molecular detection of a novel perkinsid associated with the deep‐sea clam Phreagena okutanii
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与深海蛤 Phreagena okutanii 相关的新型 Perkinsid 的分子检测

DOI:
10.1111/jeu.12917
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发表时间:
2022
影响因子:
2.2
通讯作者:
Otsubo Mayuko
Otsubo Mayuko
中科院分区:
生物学3区
文献类型:
--
作者:
Takishita Kiyotaka;Ikuta Tetsuro;Komatsu Miho;Sakaba Norika;Yoshida Takao;Otsubo Mayuko

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基于环境DNA调查,人们普遍认为,在化学合成生态系统中存在着系统发育上不同的原生生物。然而,关于与栖息在这些环境中的地方性动物相关的原生动物的知识仍然非常有限。在本研究中,我们利用聚合酶链式反应(PCR)技术,从化学合成生态系统中的代表性动物--大环文昌鱼(Phreagena Okutanii)的鳃中检测了核糖体RNA小亚基核糖体RNA(SSU RRNA)基因片段和核糖体RNA基因的内转录间隔区(ITS)。根据SSU rRNA基因的系统发育,该生物属于Perkinsus属,该属完全由感染软体动物的原生寄生虫组成。有趣的是,根据其系统发育,这种原生动物与任何已知的Perkinsus种没有亲缘关系,并在该属的辐射范围内深入分枝,因此代表了一个未被描述的物种。此外,利用荧光原位杂交技术将PCR检测到的原生质体定位于宿主文昌鱼的鳃细胞间隙。尽管这种新的深海帕金赛斯的生态意义尚不清楚,但我们目前的发现可能为研究帕金赛斯属的多样性提供重要的见解。
Based on environmental DNA surveys, it is widely held that phylogenetically diverse protists exist in chemosynthetic ecosystems. However, knowledge regarding the protists associated with the endemic animals inhabiting these environments is still very limited. In the present study, utilizing polymerase chain reaction (PCR) techniques, we detected fragments of the small subunit ribosomal RNA (SSU rRNA) gene and the internal transcribed spacer (ITS) region of the ribosomal RNA genes from a particular protist in the gills of the vesicomyid clamPhreagena okutanii(formerly described asCalyptogena okutanii), a representative animal in chemosynthetic ecosystems. Based on the phylogeny of the SSU rRNA gene, the organism in question belongs to the genusPerkinsus, which is exclusively composed of protistan parasites infecting mollusks. Intriguingly, based on the ITS phylogeny, this protist was not related to any knownPerkinsusspecies and was deeply branched within the radiation of this genus, thus represents an undescribed species. In addition, the protist detected by PCR was localized to the intercellular spaces in the gills of the host clam with fluorescence in situ hybridization. Although the ecological significance of this novel deep‐sea perkinsid remains unclear, our present findings may provide important insights into the diversity of the genusPerkinsus.
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