Molecular detection of a novel perkinsid associated with the deep‐sea clam Phreagena okutanii
Molecular detection of a novel perkinsid associated with the deep‐sea clam Phreagena okutanii
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与深海蛤 Phreagena okutanii 相关的新型 Perkinsid 的分子检测
DOI:
10.1111/jeu.12917
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发表时间:
2022
影响因子:
2.2
通讯作者:
Otsubo Mayuko
中科院分区:
文献类型:
--
作者:
Takishita Kiyotaka;Ikuta Tetsuro;Komatsu Miho;Sakaba Norika;Yoshida Takao;Otsubo Mayuko
Based on environmental DNA surveys, it is widely held that phylogenetically diverse protists exist in chemosynthetic ecosystems. However, knowledge regarding the protists associated with the endemic animals inhabiting these environments is still very limited. In the present study, utilizing polymerase chain reaction (PCR) techniques, we detected fragments of the small subunit ribosomal RNA (SSU rRNA) gene and the internal transcribed spacer (ITS) region of the ribosomal RNA genes from a particular protist in the gills of the vesicomyid clamPhreagena okutanii(formerly described asCalyptogena okutanii), a representative animal in chemosynthetic ecosystems. Based on the phylogeny of the SSU rRNA gene, the organism in question belongs to the genusPerkinsus, which is exclusively composed of protistan parasites infecting mollusks. Intriguingly, based on the ITS phylogeny, this protist was not related to any knownPerkinsusspecies and was deeply branched within the radiation of this genus, thus represents an undescribed species. In addition, the protist detected by PCR was localized to the intercellular spaces in the gills of the host clam with fluorescence in situ hybridization. Although the ecological significance of this novel deep‐sea perkinsid remains unclear, our present findings may provide important insights into the diversity of the genusPerkinsus.
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影响因子:
1.4
作者:
Beardsley, C;Knittell, K;Pernthaler, J
通讯作者:
Pernthaler, J
影响因子:
3.3
作者:
G. Reboul;D. Moreira;P. Bertolino;Alexandra Hillebrand;P. López‐García
通讯作者:
P. López‐García
影响因子:
3.4
作者:
T. Waki;M. Takahashi;Tatsuya Eki;Masato Hiasa;Kousuke Umeda;Nanae Karakawa;T. Yoshinaga
通讯作者:
T. Yoshinaga
影响因子:
2.2
作者:
Jessica A. Moss;Jie;C. Dungan;K. Reece
通讯作者:
K. Reece
影响因子:
2.2
作者:
Bower, SM;Carnegie, RB;Mak, MWS
通讯作者:
Mak, MWS