The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study.
The Potential Effect of Fucoidan on Inhibiting Epithelial-to-Mesenchymal Transition, Proliferation, and Increase in Apoptosis for Endometriosis Treatment: In Vivo and In Vitro Study.
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DOI：
10.3390/biomedicines8110528
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发表时间：
2020-11-22
期刊：
影响因子：
4.7
通讯作者：
Hsia SM
中科院分区：
文献类型：
--
作者：
Chang LC;Chiang YF;Chen HY;Huang YJ;Liu AC;Hsia SM
子宫内膜异位症在育龄妇女中很常见，其病理特征是增殖和迁移增加，以促进上皮细胞向间质转化进展（EMT）。然而，目前治疗方法有限，因此探索新的治疗药物是很重要的。因此，在本研究中，我们研究岩藻糖聚糖（FC）对子宫内膜异位症的治疗作用及其机制。采用3-（4,5-二甲基噻唑-2-酰基）-2,5-二苯基溴化四唑（MTT）法和细胞计数法测定不同浓度FC处理后子宫内膜细胞株End1/E6E7和Vk2/E6E7的细胞活力。采用创面愈合实验评估细胞迁移。在体内实验中，Balb/c雌性小鼠接受手术诱导的子宫内膜异位症治疗，随后给予不同浓度的岩藻糖聚糖治疗6周。应用高频超声成像检测后续病变的生长情况。结果表明，岩藻聚糖抑制了End1/E6E7和Vk2/E6E7细胞的生存能力和迁移能力。此外，岩藻糖聚糖可以减少子宫内膜异位症病变的体积和重量，降低血清和病变的炎症因子和血管内皮生长因子（VEGF），改善EMT增殖和凋亡相关蛋白的表达。岩藻糖聚糖首次显示出抗增殖和抗炎作用，抑制EMT进展，诱导细胞凋亡，改善子宫内膜异位症。
Endometriosis is common in reproductive-age women and its pathology is to increase proliferation and migration to enhance epithelial-to-mesenchymal transition progression (EMT). However, treatments are currently limited, so it is important to explore new therapeutic drugs. Hence, in this study, we investigate the therapeutic effect of fucoidan (FC) on the progression and mechanisms of endometriosis. The cell viability of endometrial cell lines End1/E6E7 and Vk2/E6E7 treated with different concentrations of FC were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell counting. Cell migration was evaluated using wound-healing assay. In an in vivo experiment, female Balb/c mice received surgically induced endometriosis followed by different concentrations of fucoidan for 6 weeks. High-frequency ultrasound imaging was applied to detect subsequent lesion growth. The results demonstrated that fucoidan inhibited the viability and migration ability of End1/E6E7 and Vk2/E6E7 cells. Additionally, the administration of fucoidan reduced the volume and weight of endometriotic lesions, decreased inflammatory cytokines and vascular endothelial growth factor (VEGF) of serum and lesions, and improved EMT proliferation and apoptosis-related protein expression. For the first time, fucoidan indicated anti-proliferative and anti-inflammatory effects as well as inhibited EMT progression and induced apoptosis, improving endometriosis.
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影响因子：
4.7
作者：
Bilyk O;Coatham M;Jewer M;Postovit LM
通讯作者：
Postovit LM
影响因子：
--
作者：
Chen, Hsin-Yuan;Huang, Tsui-Chin;Hsia, Shih-Min
通讯作者：
Hsia, Shih-Min
影响因子：
5.9
作者：
Kan J;Hood M;Burns C;Scholten J;Chuang J;Tian F;Pan X;Du J;Gui M
通讯作者：
Gui M
影响因子：
2.6
作者：
Machairiotis N;Stylianaki A;Dryllis G;Zarogoulidis P;Kouroutou P;Tsiamis N;Katsikogiannis N;Sarika E;Courcoutsakis N;Tsiouda T;Gschwendtner A;Zarogoulidis K;Sakkas L;Baliaka A;Machairiotis C
通讯作者：
Machairiotis C
影响因子：
7
作者：
Fernando, Ilekuttige Priyan Shanura;Dias, Mawalle Kankanamge Hasitha Madhawa;Ahn, Ginnae
通讯作者：
Ahn, Ginnae