Diversity in spatial frequency, temporal frequency, and speed tuning across mouse visual cortical areas and layers.
Diversity in spatial frequency, temporal frequency, and speed tuning across mouse visual cortical areas and layers.
复制标题
DOI：
10.1002/cne.25404
复制
发表时间：
2022-12
影响因子：
2.5
通讯作者：
Callaway, Edward M.
中科院分区：
文献类型：
--
作者：
Wang, Helen;Dey, Oyshi;Lagos, Willian N.;Callaway, Edward M.
老鼠的视觉系统由几个视觉皮质区域组成，这些区域被认为是专门用于不同的视觉特征和/或任务的。先前的研究揭示了初级视觉皮层（V1）与其他高级视觉区域，即前外侧（AL）和后内侧（PM）的差异，以及它们对空间和时间频率的调节偏好。然而，这些差异主要是用偏向于皮层表层的方法来表征的，比如双光子钙成像。很少有研究调查这些区域深层的细胞类型及其调节偏好。由于已知浅层神经元与深层神经元以及不同类型的深层神经元具有不同的前馈和反馈输入和输出，因此比较这些组的调谐偏好对于理解皮层视觉信息处理非常重要。在这项研究中，我们使用细胞外电生理学和针对两种不同的5层细胞类别的2光子钙成像来表征它们在V1， AL和PM中的调谐特性。我们发现，与浅层神经元相似，深层神经元也对不同的时空频率具有特异性，AL-V1和AL-PM之间的差异最大，而V1-PM之间的差异不明显。然而，我们注意到与之前的研究相比，深层神经元群体偏好更大范围的SFs和tf。我们还发现，脑外突出的第5层神经元比脑内突出的第5层神经元更具方向选择性。利用细胞外电生理学和基因靶向不同5层细胞的2光子钙成像，我们表征了小鼠初级视觉皮层（V1）和高级视觉皮层前外侧（AL）和后内侧（PM）区域深层（5/6层）皮层神经元的空间频率（SF）、时间频率（TF）和速度调谐。我们发现深层神经元与浅层神经元一样，在不同的视觉区域具有不同的空间和时间频率的功能。然而，我们也发现，在皮层浅层中，首选的sf和tf的范围比先前报道的要大。我们还发现，在多个视觉区域，第5层脑外投射神经元的方向调谐比第5层脑内投射神经元的方向调谐要突出得多。
The mouse visual system consists of several visual cortical areas thought to be specialized for different visual features and/or tasks. Previous studies have revealed differences between primary visual cortex (V1) and other higher visual areas, namely anterolateral (AL) and posteromedial (PM), and their tuning preferences for spatial and temporal frequency. However, these differences have primarily been characterized using methods that are biased towards superficial layers of cortex, such as 2-photon calcium imaging. Fewer studies have investigated cell types in deeper layers of these areas and their tuning preferences. Because superficial versus deep layer neurons and different types of deep layer neurons are known to have different feedforward and feedback inputs and outputs, comparing the tuning preferences of these groups is important for understanding cortical visual information processing. In this study, we used extracellular electrophysiology and 2-photon calcium imaging targeted towards two different layer 5 cell classes to characterize their tuning properties in V1, AL, and PM. We find that deep layer neurons, similar to superficial layer neurons, are also specialized for different spatial and temporal frequencies, with the strongest differences between AL-V1 and AL-PM, but not V1-PM. However, we note that the deep layer neuron populations preferred a larger range of SFs and TFs compared to previous studies. We also find that extratelencephalically projecting layer 5 neurons are more direction selective than intratelencephalically projecting layer 5 neurons. Using extracellular electrophysiology and 2-photon calcium imaging genetically targeted to different layer 5 cell classes, we characterize the spatial frequency (SF), temporal frequency (TF), and speed tuning of deep (layer 5/6) cortical neurons in mouse primary visual cortex (V1) and higher visual cortical areas anterolateral (AL) and posteromedial (PM). We find that deep layer neurons, like superficial layer neurons, are functionally specialized for different spatial and temporal frequencies in different visual areas. However, we also found a larger range of preferred SFs and TFs than previously reported in superficial cortical layers. We also find that direction tuning is much more prominent for layer 5 extratelencelphalically than layer 5 intratelencephalically projecting neurons in multiple visual areas.
登录
查看更多内容
影响因子：
25
作者：
Glickfeld, Lindsey L.;Andermann, Mark L.;Bonin, Vincent;Reid, R. Clay
通讯作者：
Reid, R. Clay
影响因子：
16.2
作者：
Kim EJ;Juavinett AL;Kyubwa EM;Jacobs MW;Callaway EM
通讯作者：
Callaway EM
提高神经元群体记录的数据质量。
影响因子：
25
作者：
Harris KD;Quiroga RQ;Freeman J;Smith SL
通讯作者：
Smith SL
影响因子：
64.8
作者：
Harris JA;Mihalas S;Hirokawa KE;Whitesell JD;Choi H;Bernard A;Bohn P;Caldejon S;Casal L;Cho A;Feiner A;Feng D;Gaudreault N;Gerfen CR;Graddis N;Groblewski PA;Henry AM;Ho A;Howard R;Knox JE;Kuan L;Kuang X;Lecoq J;Lesnar P;Li Y;Luviano J;McConoughey S;Mortrud MT;Naeemi M;Ng L;Oh SW;Ouellette B;Shen E;Sorensen SA;Wakeman W;Wang Q;Wang Y;Williford A;Phillips JW;Jones AR;Koch C;Zeng H
通讯作者：
Zeng H
影响因子：
5.3
作者：
Baker, Arielle;Kalmbach, Brian;Dembrow, Nikolai
通讯作者：
Dembrow, Nikolai