3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration.
3D Synaptic Organization of the Rat CA1 and Alterations Induced by Cocaine Self-Administration.
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DOI：
10.1093/cercor/bhaa331
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发表时间：
2021-03-05
期刊：
影响因子：
--
通讯作者：
DeFelipe J
中科院分区：
文献类型：
--
作者：
Blazquez-Llorca L;Miguéns M;Montero-Crespo M;Selvas A;Gonzalez-Soriano J;Ambrosio E;DeFelipe J
海马体在情境调节中发挥着关键作用，并被认为是可卡因成瘾大脑回路的重要组成部分。为了了解可卡因引起的该回路改变，我们使用聚焦离子束铣削/扫描电子显微镜来揭示和量化正常情况下和可卡因自我给药（SA）后大鼠 CA1 放射层神经毡的三维突触组织。大多数突触是不对称的（兴奋性的）、黄斑形状的，并且与树突棘头接触。使用可卡因-SA后，不对称和对称（抑制性）突触的大小和形状复杂性均增加，但突触密度未观察到变化。这项工作构成了第一份关于可卡因-SA 大鼠 CA1 区放射层 3D 突触组织的详细报告。我们的数据有助于阐明海马体的正常和改变的突触组织，这对于更好地理解可卡因成瘾背后的神经生物学机制至关重要。
The hippocampus plays a key role in contextual conditioning and has been proposed as an important component of the cocaine addiction brain circuit. To gain knowledge about cocaine-induced alterations in this circuit, we used focused ion beam milling/scanning electron microscopy to reveal and quantify the three-dimensional synaptic organization of the neuropil of the stratum radiatum of the rat CA1, under normal circumstances and after cocaine-self administration (SA). Most synapses are asymmetric (excitatory), macular-shaped, and in contact with dendritic spine heads. After cocaine-SA, the size and the complexity of the shape of both asymmetric and symmetric (inhibitory) synapses increased but no changes were observed in the synaptic density. This work constitutes the first detailed report on the 3D synaptic organization in the stratum radiatum of the CA1 field of cocaine-SA rats. Our data contribute to the elucidation of the normal and altered synaptic organization of the hippocampus, which is crucial for better understanding the neurobiological mechanisms underlying cocaine addiction.
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影响因子：
5.8
作者：
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影响因子：
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通讯作者：
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