Comparison of alpha-chloralose, medetomidine and isoflurane anesthesia for functional connectivity mapping in the rat.
Comparison of alpha-chloralose, medetomidine and isoflurane anesthesia for functional connectivity mapping in the rat.
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DOI：
10.1016/j.mri.2010.03.007
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发表时间：
2010-09
影响因子：
2.5
通讯作者：
Keilholz, Sheila D.
中科院分区：
文献类型：
--
作者：
Williams, Kathleen A.;Magnuson, Matthew;Majeed, Waqas;LaConte, Stephen M.;Peltier, Scott J.;Hu, Xiaoping;Keilholz, Sheila D.
基于低频血氧水平依赖性功能磁共振成像（BOLD fMRI）信号波动的功能连接测量已成为研究人类自发脑活动的广泛使用的工具。然而，神经活动、血液动力学反应和MRI信号波动之间的精确关系仍然未知。最近几个研究小组的工作表明，在麻醉的大鼠中可以检测到BOLD信号中的相关低频波动-这是阐明这种关系的第一步。在这项初步工作的基础上，通过这项研究，我们证明了在大鼠中观察到的功能连接在很大程度上取决于所使用的麻醉类型。使用高时间分辨率成像序列（确保生理噪声污染最小）显示了α-氯醛糖、美托咪定或异氟烷麻醉大鼠的自发波动功率谱和基于互相关的连接图。结果显示，与α-氯醛糖或美托咪定麻醉的大鼠相比，异氟烷麻醉的大鼠中的局部相关性较低。这些实验强调了使用不同类型的麻醉来探索BOLD信号的基本生理关系的实用性，并表明有助于功能连接的机制涉及神经活动、神经血管耦合和血管反应性变化之间的复杂关系。
Functional connectivity measures based upon low-frequency blood-oxygenation-level-dependent functional magnetic resonance imaging (BOLD fMRI) signal fluctuations have become a widely used tool for investigating spontaneous brain activity in humans. Still unknown, however, is the precise relationship between neural activity, the hemodynamic response and fluctuations in the MRI signal. Recent work from several groups had shown that correlated low-frequency fluctuations in the BOLD signal can be detected in the anesthetized rat — a first step toward elucidating this relationship. Building on this preliminary work, through this study, we demonstrate that functional connectivity observed in the rat depends strongly on the type of anesthesia used. Power spectra of spontaneous fluctuations and the cross-correlation-based connectivity maps from rats anesthetized with α-chloralose, medetomidine or isoflurane are presented using a high-temporal-resolution imaging sequence that ensures minimal contamination from physiological noise. The results show less localized correlation in rats anesthetized with isoflurane as compared with rats anesthetized with α-chloralose or medetomidine. These experiments highlight the utility of using different types of anesthesia to explore the fundamental physiological relationships of the BOLD signal and suggest that the mechanisms contributing to functional connectivity involve a complicated relationship between changes in neural activity, neurovascular coupling and vascular reactivity.
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DOI：
10.1073/pnas.95.26.15741
发表时间：
1998-12-22
影响因子：
11.1
作者：
Kleinfeld, D;Mitra, PP;Denk, W
通讯作者：
Denk, W
DOI：
10.1073/pnas.0705791104
发表时间：
2007-11-13
影响因子：
11.1
作者：
Lu, Hanbing;Zuo, Yantao;Stein, Elliot A.
通讯作者：
Stein, Elliot A.
影响因子：
3.3
作者：
HU, XP;LE, TH;ERHARD, P
通讯作者：
ERHARD, P
影响因子：
2.5
作者：
Kiviniemi, VJ;Haanpää, H;Tervonen, O
通讯作者：
Tervonen, O
影响因子：
3.3
作者：
Keilholz, SD;Silva, AC;Koretsky, AP
通讯作者：
Koretsky, AP