A new co-culture method for identifying synaptic adhesion molecules involved in synapse formation
A new co-culture method for identifying synaptic adhesion molecules involved in synapse formation
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一种新的共培养方法，用于识别参与突触形成的突触粘附分子
DOI：
10.1007/s41048-019-0084-4
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Xiaofei Yang
中科院分区：
文献类型：
--
作者：
Wei Jiang;Jihong Gong;Yi Rong;Xiaofei Yang
突触的正常形成对于大脑功能至关重要。人们认为突触细胞黏附分子（CAMs）在突触形成过程的起始阶段发挥着关键作用。人工突触形成检测法，其中涉及非神经元……
（你提供的英文内容不完整，最后“non - neuronal”后应该还有内容，完整内容翻译会更准确通顺。）
The proper formation of synapses is essential for brain function. Synaptic cell adhesion molecules (CAMs) are thought to play essential roles in the initiation of the synapse formation process. The artificial synapse formation assay, in which non-neuronal cells and neurons are co-cultured, has been shown to be a powerful system for screening CAMs. However, controlling a large number of cell pools in co-culture is complicated, creating a potential barrier for high-throughput screening. This protocol describes a new co-culture method in which cDNA plasmid is transfected into human embryonic kidney 293T cells using polyetherimide 24 h after cells were mixed with neurons, and immunostaining and confocal imaging are employed for analyzing synaptogenesis. This optimized method is simpler and easier to perform than the traditional method for the examination of the synaptogenic activities of individual cell-surface proteins in isolation, and provides an unbiased screening platform for synaptogenic proteins.
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影响因子：
25
作者：
Kim, Seho;Burette, Alain;Kim, Eunjoon
通讯作者：
Kim, Eunjoon
影响因子：
34.7
作者：
Pereda, Alberto E.
通讯作者：
Pereda, Alberto E.
影响因子：
56.9
作者：
Biederer, T;Sara, Y;Südhof, TC
通讯作者：
Südhof, TC
鉴定 O 型蛋白酪氨酸磷酸酶受体 (PTPRO) 作为促进突触形成的突触粘附分子。
影响因子：
5.3
作者：
Jiang, Wei;Wei, Mengping;Zhang, Chen
通讯作者：
Zhang, Chen
影响因子：
16.2
作者：
Südhof TC;Malenka RC
通讯作者：
Malenka RC