BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
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BlyS 因缺氧而上调并促进人乳腺癌细胞迁移
DOI：
10.1186/1756-9966-31-31
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发表时间：
2012-03-31
期刊：
影响因子：
--
通讯作者：
Yuan S
中科院分区：
文献类型：
--
作者：
Zhu J;Sun L;Lin S;Zhao R;Zhou L;Fang D;Chen L;Liu J;Shi W;Zhang L;Yuan S
背景B淋巴细胞刺激因子（BLyS）在恶性B细胞存活和维持正常B细胞发育和稳态中的作用已被广泛研究。然而，BLyS对乳腺癌进展的影响仍不清楚。本研究旨在探讨缺氧对BLyS的调节作用、BLyS对细胞迁移的影响及其可能的分子机制。MethodsIn this study，我们通过transwell法检测了BLyS在人乳腺癌细胞迁移中的作用。我们还探讨了BLyS及其受体是否在人乳腺癌细胞系中表达的免疫荧光和Western印迹。然后采用真实的时间PCR和Western Blotting检测BLyS在常氧和低氧条件下的表达水平。通过Western Blotting、免疫荧光、transwell和luciferase assay. ResultsAccordingto我们的研究，BlyS的表达水平在缺氧条件下的人乳腺癌细胞系中增加。该蛋白的上调导致NF-κ B p65的活化和核转位。我们还发现，在BLyS和Akt的磷酸化抑制的存在下，迁移细胞的数量增加减弱增强migrationresponse.ConclusionsIt建议，更好地了解BLyS，免疫增强剂，可能提供一个潜在的治疗靶点，用于治疗人类乳腺癌。此外，BLyS促进乳腺癌细胞迁移，强调了适当应用免疫增强剂治疗癌症的必要性。
BackgroundThe role of B Lymphocyte Stimulator (BLyS) in the survival of malignant B cells and the maintenance of normal B cell development and homeostasis has been intensively studied in the literature. However, the influence of BLyS on breast cancer progression remains unclear. The study aimed to investigate the effect of hypoxia on BLyS regulation, cell migratory response to BLyS and the possible molecular mechanisms.MethodsIn this study, we examined the role of BLyS in the migration of human breast cancer cells by transwell assay. We also explored whether BLyS and its receptors expressed in human breast cancer cell lines by immunofluorescence and Western Blotting. Then we detected the expression level of BLyS in both normoxic and hypoxic conditions by real time-PCR and Western Blotting. Pathways involved were confirmed by Western Blotting, immunofluorescence, transwell assay and luciferase assay.ResultsAccording to our study, the expression level of BlyS was increased in human breast cancer cell lines in hypoxic conditions. Up-regulation of this protein led to activation and nuclear translocation of NF-kappa B p65. We also found that the number of migrated cells was increased in the presence of BLyS and inhibition of phosphorylation of Akt attenuated the enhanced migratory response.ConclusionsIt suggested that better understanding of BLyS, an immunopotentiator, may offer a potential therapeutic target for the treatment of human breast cancers. In addition, BLyS promoted breast cancer cells migration, underscoring the necessity of appropriate applications of immunopotentiators to cancer treatment.
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影响因子：
4.8
作者：
Gonzalez-Perez RR;Xu Y;Guo S;Watters A;Zhou W;Leibovich SJ
通讯作者：
Leibovich SJ
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影响因子：
3.8
作者：
Pelekanou V;Kampa M;Kafousi M;Darivianaki K;Sanidas E;Tsiftsis DD;Stathopoulos EN;Tsapis A;Castanas E
通讯作者：
Castanas E
影响因子：
3.6
作者：
Mohamed, KM;Le, A;Messadi, DV
通讯作者：
Messadi, DV
影响因子：
3.8
作者：
Yu, YY;Li, Q;Zhu, ZG
通讯作者：
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影响因子：
11.1
作者：
Miguel Lopez-Novoa, Jose;Angela Nieto, M.
通讯作者：
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