Ameliorative potential of desalted Salicornia europaea L. extract in multifaceted Alzheimer's-like scopolamine-induced amnesic mice model.
Ameliorative potential of desalted Salicornia europaea L. extract in multifaceted Alzheimer's-like scopolamine-induced amnesic mice model.
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DOI：
10.1038/s41598-018-25381-0
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发表时间：
2018-05-08
影响因子：
4.6
通讯作者：
Choi DK
中科院分区：
文献类型：
--
作者：
Karthivashan G;Park SY;Kweon MH;Kim J;Haque ME;Cho DY;Kim IS;Cho EA;Ganesan P;Choi DK
盐角草Salicornia europaea L. (SE)植物是一种盐生植物，已被广泛用作调味蔬菜，最近有报道称它可以对抗与氧化和炎症应激有关的慢性疾病。在这项研究中，我们进行了初步的植物化学分析，在体外生化测试和色谱分析脱盐和酶消化SE乙醇提取物（SE-EE）。随后，我们评估了SE-EE的抗神经炎症和改善潜力，在脂多糖造成的BV-2小胶质细胞和东莨菪碱诱导的健忘症C57/BL 6 N小鼠，分别。SE-EE具有相当多的多酚和黄酮类化合物，据推测，这些物质是提高其生物功效的原因。SE-EE剂量依赖性地减弱BV-2细胞中LPS诱导的炎症，显著抑制行为/认知障碍，剂量依赖性地调节胆碱能功能，抑制氧化应激标志物，调节炎性细胞因子/相关蛋白表达，并有效改善东莨菪碱给药小鼠中的p-CREB/BDNF水平、神经发生（DCX染色）、神经元增殖（Ki 67染色）。因此，SE-EE提取物显示出有希望的多因子疾病改善活性，并且可以进一步开发为有效的功能性食品、候选药物或治疗神经炎症介导的疾病的补充疗法。
The Salicornia europaea L. (SE) plant is a halophyte that has been widely consumed as a seasoned vegetable, and it has been recently reported to counteract chronic diseases related to oxidative and inflammatory stress. In this study, we performed an initial phytochemical analysis with in vitro biochemical tests and chromatographic profiling of desalted and enzyme-digested SE ethanol extract (SE-EE). Subsequently, we evaluated the anti-neuroinflammatory and ameliorative potential of SE-EE in LPS-inflicted BV-2 microglial cells and scopolamine-induced amnesic C57/BL6N mice, respectively. SE-EE possess considerable polyphenols and flavonoids that are supposedly responsible to improve its bio-efficacy. SE-EE dose-dependently attenuated LPS-induced inflammation in BV-2 cells, significantly repressed behavioural/cognitive impairment, dose-dependently regulated the cholinergic function, suppressed oxidative stress markers, regulated inflammatory cytokines/associated proteins expression and effectively ameliorated p-CREB/BDNF levels, neurogenesis (DCX stain), neuron proliferation (Ki67 stain) in scopolamine-administered mice. Thus, SE-EE extract shows promising multifactorial disease modifying activities and can be further developed as an effective functional food, drug candidate, or supplemental therapy to treat neuroinflammatory mediated disorders.
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影响因子：
6
作者：
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通讯作者：
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