Involvement of multiple distinct Bordetella receptor proteins in the utilization of iron liberated from transferrin by host catecholamine stress hormones.
Involvement of multiple distinct Bordetella receptor proteins in the utilization of iron liberated from transferrin by host catecholamine stress hormones.
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DOI：
10.1111/j.1365-2958.2012.08032.x
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发表时间：
2012-05
影响因子：
3.6
通讯作者：
Suhadolc RJ
中科院分区：
文献类型：
--
作者：
Armstrong SK;Brickman TJ;Suhadolc RJ
支气管败血博德特氏菌是一种病原体，可以利用其天然的碱铁蛋白铁载体系统获取铁，但也可以通过 BfeA 外膜受体使用儿茶酚异铁载体肠杆菌素。 bfeA 的转录受到需要肠杆菌素诱导的调节因子的正向控制。儿茶酚胺激素还诱导 bfeA 转录，支气管败血博德氏菌可以利用儿茶酚胺去甲肾上腺素在转铁蛋白上生长。在这项研究中，支气管败血症博德氏菌在没有铁载体的情况下使用儿茶酚胺从转铁蛋白和乳铁蛋白中获取铁。在铁载体存在的情况下，去甲肾上腺素增强了支气管败血杆菌对转铁蛋白的利用，以及体外铁载体的功能。遗传分析确定 BfrA、BfrD 和 BfrE 为 TonB 依赖性外膜儿茶酚胺受体。发现 BfeA 肠杆菌素受体不直接参与儿茶酚胺的利用；然而，BfrA、BfrD 和 BfrE 儿茶酚胺受体可以作为肠杆菌素及其降解产物 2,3-二羟基苯甲酸的受体。因此，肠杆菌素依赖性和儿茶酚胺依赖性转铁蛋白利用之间存在功能联系。这项研究描述了支气管败血杆菌从转铁蛋白中摄取铁的新机制，该机制使用宿主应激激素，不仅将铁直接递送至儿茶酚胺受体，而且还通过充当铁梭来增强铁载体活性。
Bordetella bronchiseptica is a pathogen that can acquire iron using its native alcaligin siderophore system, but can also use the catechol xenosiderophore enterobactin via the BfeA outer membrane receptor. Transcription of bfeA is positively controlled by a regulator that requires induction by enterobactin. Catecholamine hormones also induce bfeA transcription and B. bronchiseptica can use the catecholamine norepinephrine for growth on transferrin. In this study, B. bronchiseptica was shown to use catecholamines to obtain iron from both transferrin and lactoferrin in the absence of siderophore. In the presence of siderophore, norepinephrine augmented transferrin utilization by B. bronchiseptica, as well as siderophore function in vitro. Genetic analysis identified BfrA, BfrD and BfrE as TonB dependent outer membrane catecholamine receptors. The BfeA enterobactin receptor was found to not be involved directly in catecholamine utilization; however, the BfrA, BfrD and BfrE catecholamine receptors could serve as receptors for enterobactin and its degradation product 2,3-dihydroxybenzoic acid. Thus, there is a functional link between enterobactin-dependent and catecholamine-dependent transferrin utilization. This investigation characterizes a new B. bronchiseptica mechanism for iron uptake from transferrin that uses host stress hormones that not only deliver iron directly to catecholamine receptors, but also potentiate siderophore activity by acting as iron shuttles.
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影响因子：
3.1
作者：
Coulanges, V;Andre, P;Vidon, DJM
通讯作者：
Vidon, DJM
影响因子：
4.1
作者：
Devanur, Lakshmi D.;Evans, Robert W.;Hider, Robert C.
通讯作者：
Hider, Robert C.
DOI：
10.1099/00221287-143-1-135
发表时间：
1997-01-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Beall, B;Hoenes, T
通讯作者：
Hoenes, T
影响因子：
2.6
作者：
de Rossi, BNP;Friedman, LE;Franco, MA
通讯作者：
Franco, MA
影响因子：
3.2
作者：
Anderson, Mark T.;Armstrong, Sandra K.
通讯作者：
Armstrong, Sandra K.