Stay or go? Neuronal activity in medial frontal cortex during a voluntary tactile preference task in head-fixed mice.
Stay or go? Neuronal activity in medial frontal cortex during a voluntary tactile preference task in head-fixed mice.
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去还是留？头部固定小鼠在主动触觉偏好任务中内侧额叶皮层的神经元活动。
DOI：
10.1016/j.ceca.2021.102388
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发表时间：
2021-06
期刊：
影响因子：
4
通讯作者：
Aldridge GM
中科院分区：
文献类型：
--
作者：
Keyes AL;Kim YC;Bosch PJ;Usachev YM;Aldridge GM
移动的决定受到感官、注意力和动机线索的影响。其中一个线索是触觉输入的质量，有毒或不愉快的感觉会导致动物远离线索。大脑皮层对疼痛和不愉快感觉的处理涉及多个大脑区域，尽管大脑区域在远离不愉快刺激的自发性运动而非反射性运动中所起的具体作用尚不清楚。在此，我们重点研究了次级运动皮层的内侧分支，该分支被提出将感觉和情境线索与运动动作联系起来，并测试了其在厌恶触觉线索背景下控制自主运动的作用。我们设计了一种新颖的3d打印触觉平台，由无害（网格）和轻度有害（尖刺）表面（占总面积的50:50%）组成，可以通过双光子成像在头部固定的小鼠的感觉偏好任务中监测内侧额叶皮层的神经元活动。我们发现，自由活动的小鼠在刺状表面上花费的时间明显减少，这种偏好通过局部麻醉消除。在神经元水平上，单个神经元对所遇到的触觉表面有不同的调节。在群体水平上，研究人员分析了神经元活动与小鼠选择“停止”或“离开”特定触觉表面以及不停止地“切换”表面的事件之间的关系。值得注意的是，这三种场景中的每一种都显示出网格和尖刺触觉表面之间的种群活动差异很大。总的来说，这些数据提供了触觉质量编码于内侧额叶皮层的证据。在这项研究中开创的任务提供了一个有价值的工具，以更好地评估老鼠的伤害感觉和疼痛模型，使用一个自愿的任务，允许同时记录偏好和选择。
The decision to move is influenced by sensory, attentional, and motivational cues. One such cue is the quality of the tactile input, with noxious or unpleasant sensations causing an animal to move away from the cue. Processing of painful and unpleasant sensation in the cortex involves multiple brain regions, although the specific role of the brain areas involved in voluntary, rather than reflexive movement away from unpleasant stimuli is not well understood. Here, we focused on the medial subdivision of secondary motor cortex, which is proposed to link sensory and contextual cues to motor action, and tested its role in controlling voluntary movement in the context of an aversive tactile cue. We designed a novel, 3D-printed tactile platform consisting of innocuous (grid) and mildly noxious (spiked) surfaces (50:50% of total area), which enabled monitoring neuronal activity in the medial frontal cortex by two-photon imaging during a sensory preference task in head-fixed mice. We found that freely moving mice spent significantly less time on a spiked-surface, and that this preference was eliminated by administration of a local anesthetic. At the neuronal level, individual neurons were differentially modulated specific to the tactile surface encountered. At the population level, the neuronal activity was analyzed in relation to the events where mice chose to “stop-on” or “go-from” a specific tactile surface and when they “switched” surfaces without stopping. Notably, each of these three scenarios showed population activity that differed significantly between the grid and spiked tactile surfaces. Collectively, these data provide evidence that tactile quality is encoded within medial frontal cortex. The task pioneered in this study provides a valuable tool to better evaluate mouse models of nociception and pain, using a voluntary task that allows simultaneous recording of preference and choice.
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运动灵量和强度取决于注意力控制系统的完整性。
DOI：
10.1073/pnas.1715617115
发表时间：
2018-01-16
影响因子：
11.1
作者：
Rinne P;Hassan M;Fernandes C;Han E;Hennessy E;Waldman A;Sharma P;Soto D;Leech R;Malhotra PA;Bentley P
通讯作者：
Bentley P
DOI：
10.1016/j.cub.2016.11.029
发表时间：
2017-01-09
期刊：
Current biology : CB
影响因子：
--
作者：
Kim YC;Han SW;Alberico SL;Ruggiero RN;De Corte B;Chen KH;Narayanan NS
通讯作者：
Narayanan NS
影响因子：
4.8
作者：
Deuis JR;Dvorakova LS;Vetter I
通讯作者：
Vetter I
影响因子：
15.9
作者：
Barthas F;Kwan AC
通讯作者：
Kwan AC
影响因子：
25
作者：
Navratilova, Edita;Porreca, Frank
通讯作者：
Porreca, Frank