Analysis of the unexplored features of rrs (16S rDNA) of the Genus Clostridium.
Analysis of the unexplored features of rrs (16S rDNA) of the Genus Clostridium.
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DOI：
10.1186/1471-2164-12-18
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发表时间：
2011-01-11
期刊：
影响因子：
4.4
通讯作者：
Huma N
中科院分区：
文献类型：
--
作者：
Kalia VC;Mukherjee T;Bhushan A;Joshi J;Shankar P;Huma N
基于RRS(16S RDNA)测序的细菌分类和系统发育正在大力研究。事实上，有人指出，新的生物学发现是由海量数据集的比较和整合推动的。尽管有1,237,963个条目的大量RRS测序数据，但这种分析总是需要其他基因的补充。需要在RRS系统发育边界划分一个分类群或属内的遗传可变性，并发现这些基本特征，这将使细菌能够自然地落入其中。在大型细菌群落中，梭状芽孢杆菌代表着一个由大约110个物种组成的大属，具有重要的生物技术和医学意义。某些梭状芽胞杆菌菌株产生一些最致命的毒素，造成严重的经济损失。我们的目标是这个属，因为它具有高度的遗传多样性，这使得现有的分子方法无法进行准确的分型。分析了110种梭状芽孢杆菌的765个RRS序列(>；1200个核苷酸，NTS)。根据15个种的404个RRS序列，我们建立了物种特有的：(I)系统发育框架，(Ii)特征(30nts)和(Iii)硅胶限制性内切酶(14个II型RES)的酶切模式。这些方法可以：(1)对95株梭状芽胞杆菌进行种水平鉴定。(Ii)84株新梭状芽孢杆菌的鉴定。以及(Iii)以小种群为代表的梭状芽胞杆菌物种数量的潜在减少。这种综合方法非常敏感，可以很容易地扩展为一种分子工具，用于诊断和分类鉴定任何对食品工业和卫生服务重要的微生物。由于快速和正确的识别可以更快地诊断和治疗，这可能会减少经济损失和死亡率。
Bacterial taxonomy and phylogeny based on rrs (16S rDNA) sequencing is being vigorously pursued. In fact, it has been stated that novel biological findings are driven by comparison and integration of massive data sets. In spite of a large reservoir of rrs sequencing data of 1,237,963 entries, this analysis invariably needs supplementation with other genes. The need is to divide the genetic variability within a taxa or genus at their rrs phylogenetic boundaries and to discover those fundamental features, which will enable the bacteria to naturally fall within them. Within the large bacterial community, Clostridium represents a large genus of around 110 species of significant biotechnological and medical importance. Certain Clostridium strains produce some of the deadliest toxins, which cause heavy economic losses. We have targeted this genus because of its high genetic diversity, which does not allow accurate typing with the available molecular methods. Seven hundred sixty five rrs sequences (> 1200 nucleotides, nts) belonging to 110 Clostridium species were analyzed. On the basis of 404 rrs sequences belonging to 15 Clostridium species, we have developed species specific: (i) phylogenetic framework, (ii) signatures (30 nts) and (iii) in silico restriction enzyme (14 Type II REs) digestion patterns. These tools allowed: (i) species level identification of 95 Clostridium sp. which are presently classified up to genus level, (ii) identification of 84 novel Clostridium spp. and (iii) potential reduction in the number of Clostridium species represented by small populations. This integrated approach is quite sensitive and can be easily extended as a molecular tool for diagnostic and taxonomic identification of any microbe of importance to food industries and health services. Since rapid and correct identification allows quicker diagnosis and consequently treatment as well, it is likely to lead to reduction in economic losses and mortality rates.
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影响因子：
5.6
作者：
Carere CR;Sparling R;Cicek N;Levin DB
通讯作者：
Levin DB
影响因子：
5.4
作者：
Cocolin, L;Innocente, N;Comi, G
通讯作者：
Comi, G
影响因子：
56.9
作者：
FOX, GE;STACKEBRANDT, E;WOESE, CR
通讯作者：
WOESE, CR
影响因子：
9.4
作者：
Asha, N. J.;Tompkins, D.;Wilcox, M. H.
通讯作者：
Wilcox, M. H.
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE