Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.
Antisense expression of the fasciclin-like arabinogalactan protein FLA6 gene in Populus inhibits expression of its homologous genes and alters stem biomechanics and cell wall composition in transgenic trees.
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杨属中成束蛋白样阿拉伯半乳聚糖蛋白FLA6基因的反义表达抑制其同源基因的表达并改变转基因树中的茎生物力学和细胞壁组成
DOI：
10.1093/jxb/eru479
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发表时间：
2015-03
影响因子：
6.9
通讯作者：
Zhang H
中科院分区：
文献类型：
--
作者：
Wang H;Jiang C;Wang C;Yang Y;Yang L;Gao X;Zhang H
Fasciclin-like arabinogalactan蛋白是一类细胞壁糖蛋白，在维持杨树茎生物力学和细胞壁组成方面发挥重要作用。 Fasciclin-like arabinogalactan proteins（FLAs）在拟南芥根、茎和种子的生长发育中起重要作用。然而，它们在木本植物中的生物学功能在很大程度上是未知的。本研究对PtFLA 6在白杨中的可能功能进行了研究。实时荧光定量PCR、PtFLA 6-YFP融合蛋白亚细胞定位、Western blotting和免疫组织化学分析表明，PtFLA 6基因在成熟茎的木质部特异表达，PtFLA 6蛋白在植物细胞中普遍存在，主要积累在茎木质部纤维中。在白杨杂种无性系白杨×白杨中，PtFLA 6反义表达降低了PtFLA 6及其同源基因的转录水平。表现出显着减少的转录PtFLAs的转基因植物积累较少的PtFLA 6和阿拉伯半乳聚糖蛋白比非转基因植物，导致茎弯曲强度和刚度降低。进一步的研究表明，转基因植物茎生物力学的改变可能是由于木质部中纤维素和木质素成分的减少。此外，一些参与细胞壁生物合成的木质部特异性基因在这些转基因植物中表达下调。所有这些结果表明，工程PtFLA 6及其同源物的表达可以通过影响树木细胞壁组成来调节茎的机械性能。
Fasciclin-like arabinogalactan proteins, a class of cell wall glycoprotein, function in maintaining stem biomechanics and cell wall composition in Populus. Fasciclin-like arabinogalactan proteins (FLAs) play important roles in the growth and development of roots, stems, and seeds in Arabidopsis. However, their biological functions in woody plants are largely unknown. In this work, we investigated the possible function of PtFLA6 in poplar. Quantitative real-time PCR, PtFLA6–yellow fluorescent protein (YFP) fusion protein subcellular localization, Western blotting, and immunohistochemical analyses demonstrated that the PtFLA6 gene was expressed specifically in the xylem of mature stem, and PtFLA6 protein was distributed ubiquitous in plant cells and accumulated predominantly in stem xylem fibres. Antisense expression of PtFLA6 in the aspen hybrid clone Poplar davidiana×Poplar bolleana reduced the transcripts of PtFLA6 and its homologous genes. Transgenic plants that showed a significant reduction in the transcripts of PtFLAs accumulated fewer PtFLA6 and arabinogalactan proteins than did the non-transgenic plants, leading to reduced stem flexural strength and stiffness. Further studies revealed that the altered stem biomechanics of transgenic plants could be attributed to the decreased cellulose and lignin composition in the xylem. In addition expression of some xylem-specific genes involved in cell wall biosynthesis was downregulated in these transgenic plants. All these results suggest that engineering the expression of PtFLA6 and its homologues could modulate stem mechanical properties by affecting cell wall composition in trees.
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影响因子：
4
作者：
Lorenz, WW;Dean, JFD
通讯作者：
Dean, JFD
影响因子：
6.4
作者：
No, EG;Loopstra, CA
通讯作者：
Loopstra, CA
影响因子：
7.2
作者：
MacMillan, Colleen P.;Mansfield, Shawn D.;Southerton, Simon G.
通讯作者：
Southerton, Simon G.
影响因子：
7.2
作者：
Levitin, Bella;Richter, Dganit;Zik, Moriyah
通讯作者：
Zik, Moriyah
DOI：
10.1073/pnas.94.15.8255
发表时间：
1997-07-22
影响因子：
11.1
作者：
MacKay, JJ;OMalley, DM;Sederoff, RR
通讯作者：
Sederoff, RR