Hypothalamus-olfactory system crosstalk: orexin a immunostaining in mice.
Hypothalamus-olfactory system crosstalk: orexin a immunostaining in mice.
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DOI：
10.3389/fnana.2012.00044
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发表时间：
2012
影响因子：
2.9
通讯作者：
Lopez-Mascaraque L
中科院分区：
文献类型：
--
作者：
Gascuel J;Lemoine A;Rigault C;Datiche F;Benani A;Penicaud L;Lopez-Mascaraque L
众所周知，嗅觉影响食物摄入，相反，个体的营养状况调节嗅觉敏感性。然而，这种关系的神经元相关性以及嗅球和下丘脑之间的联系仍然知之甚少。本报告的目的是分析嗅球和下丘脑之间的关系，重点是食欲素A免疫染色，下丘脑神经肽，被认为在睡眠/觉醒状态中发挥作用。有趣的是，食欲素A也被描述为食物摄入刺激剂。这种效应可能部分是由于刺激了嗅球通路。在大鼠中，食欲素阳性细胞严格集中在外侧下丘脑，而它们的投射几乎侵入整个大脑，包括嗅觉系统。因此，食欲素似乎是一个很好的候选人发挥关键作用，在连接嗅觉和下丘脑通路。到目前为止，食欲素已经在大鼠中被描述，然而，仍然缺乏关于其在成年和发育中小鼠大脑中表达的信息。在这种情况下，我们重新审视了食欲素A模式在成年和发展中的小鼠使用免疫组织学方法和共聚焦显微镜。除了微小的差异外，小鼠中的食欲素A免疫染色与在大鼠中观察到的那些具有许多特征。在嗅球中，尽管有很少的食欲素投射，但它们到达嗅球的所有不同层。与主嗅球中食欲素投射的存在相反，在副嗅球中几乎没有发现食欲素投射。食欲素A的发育表达支持食欲素表达仅在出生后出现的假设。
It is well known that olfaction influences food intake, and conversely, that an individual’s nutritional status modulates olfactory sensitivity. However, what is still poorly understood is the neuronal correlate of this relationship, as well as the connections between the olfactory bulb and the hypothalamus. The goal of this report is to analyze the relationship between the olfactory bulb and hypothalamus, focusing on orexin A immunostaining, a hypothalamic neuropeptide that is thought to play a role in states of sleep/wakefulness. Interestingly, orexin A has also been described as a food intake stimulator. Such an effect may be due in part to the stimulation of the olfactory bulbar pathway. In rats, orexin positive cells are concentrated strictly in the lateral hypothalamus, while their projections invade nearly the entire brain including the olfactory system. Therefore, orexin appears to be a good candidate to play a pivotal role in connecting olfactory and hypothalamic pathways. So far, orexin has been described in rats, however, there is still a lack of information concerning its expression in the brains of adult and developing mice. In this context, we revisited the orexin A pattern in adult and developing mice using immunohistological methods and confocal microscopy. Besides minor differences, orexin A immunostaining in mice shares many features with those observed in rats. In the olfactory bulb, even though there are few orexin projections, they reach all the different layers of the olfactory bulb. In contrast to the presence of orexin projections in the main olfactory bulb, almost none have been found in the accessory olfactory bulb. The developmental expression of orexin A supports the hypothesis that orexin expression only appears post-natally.
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影响因子：
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作者：
Hahn JD;Swanson LW
通讯作者：
Swanson LW
影响因子：
3.6
作者：
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通讯作者：
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作者：
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Julliard, A. K.
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通讯作者：
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影响因子：
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作者：
Horvath, TL;Gao, XB
通讯作者：
Gao, XB