Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster.
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DOI：
10.1111/j.1365-2958.2011.07753.x
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发表时间：
2011-08
影响因子：
3.6
通讯作者：
Setlow P
中科院分区：
文献类型：
--
作者：
Griffiths KK;Zhang J;Cowan AE;Yu J;Setlow P
休眠细菌的孢子对环境的损害有很强的抵抗力，是各种疾病的载体。然而，孢子不能引起疾病，除非它们发芽并成为营养细胞。萌发起始的分子细节尚不清楚，但萌发早期必需的蛋白质，如营养萌发受体（GR）和GerD，位于孢子内膜。在这项研究中，我们研究这些萌发蛋白是如何组织在休眠的枯草芽孢杆菌孢子表达荧光蛋白融合，至少部分功能和观察孢子荧光显微镜。我们发现，GR和GerD共定位主要是一个单一的集群在休眠孢子，让人想起组织中的大肠杆菌的化学感受器信号复合物。GR需要它们的所有亚基以及GerD用于聚类，并且还需要将二酰基甘油添加到GerD和GR的C蛋白亚基。然而，不同的GR彼此独立地聚类，并且GerD在所有GR不存在的情况下形成聚类。我们预测，集群代表孢子内膜中的功能性萌发单位或“germinosome”，这对于快速和合作响应营养物质是必要的，因为已知阻止营养萌发的条件也会破坏蛋白质集群。
Dormant bacterial spores are extraordinarily resistant to environmental insults and are vectors of various illnesses. However, spores cannot cause disease unless they germinate and become vegetative cells. The molecular details of initiation of germination are not understood, but proteins essential in early stages of germination, such as nutrient germinant receptors (GRs) and GerD, are located in the spore inner membrane. In this study, we examine how these germination proteins are organized in dormant Bacillus subtilis spores by expressing fluorescent protein fusions that were at least partially functional and observing spores by fluorescence microscopy. We show that GRs and GerD colocalize primarily to a single cluster in dormant spores, reminiscent of the organization of chemoreceptor signalling complexes in Escherichia coli. GRs require all their subunits as well as GerD for clustering, and also require diacylglycerol addition to GerD and GRs’ C protein subunits. However, different GRs cluster independently of each other, and GerD forms clusters in the absence of all the GRs. We predict that the clusters represent a functional germination unit or ‘germinosome’ in the spore inner membrane that is necessary for rapid and cooperative response to nutrients, as conditions known to block nutrient germination also disrupt the protein clusters.
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影响因子：
10.5
作者：
Lopez, Daniel;Kolter, Roberto
通讯作者：
Kolter, Roberto
DOI：
10.1073/pnas.0306859101
发表时间：
2004-05-18
影响因子：
11.1
作者：
Cowan, AE;Olivastro, EM;Setlow, P
通讯作者：
Setlow, P
影响因子：
3.2
作者：
Cooper, Gareth R.;Moir, Anne
通讯作者：
Moir, Anne
影响因子：
3.2
作者：
Ghosh, Sonali;Setlow, Peter
通讯作者：
Setlow, Peter
影响因子：
3.2
作者：
Igarashi, T;Setlow, B;Setlow, P
通讯作者：
Setlow, P