Spatiotemporal and Transcriptional Characterization on Tanshinone Initial Synthesis in Salvia miltiorrhiza Roots.
Spatiotemporal and Transcriptional Characterization on Tanshinone Initial Synthesis in Salvia miltiorrhiza Roots.
复制标题
丹参根中丹参酮初始合成的时空和转录特征
DOI：
10.3390/ijms232113607
复制
发表时间：
2022-11-06
影响因子：
5.6
通讯作者：
Song, Zhenqiao
中科院分区：
文献类型：
--
作者：
Lin, Caicai;Zhang, Lin;Zhang, Xia;Wang, Xin;Wang, Chaoyang;Zhang, Yufeng;Wang, Jianhua;Li, Xingfeng;Song, Zhenqiao
丹参酮是中药用于治疗心血管等疾病的丹参（Salvia miltiorrhiza Bunge）的生物活性成分，在丹参根周合成并积累。然而，关于丹参酮合成的初始阶段，以及根茎结构和基因表达特征，目前尚无相关报道。本研究旨在通过从空间和时间方面表征这些生物活性原理在根系发育早期的生物合成和积累可能存在的差异，为这些生物活性原理如何开始合成提供新的见解。采用萌发后65 d （DAGs）监测的方法，对丹参根系形态特征和丹参酮含量进行了详细的研究。ONT转录组测序用于研究基因表达模式。丹参贮藏主根的茎周在约30个dag上初步合成丹参酮。初步确定了丹参酮合成的三个关键阶段：制备阶段、初始合成阶段和连续快速合成阶段。前两个阶段主根差异最小，差异表达基因（DEGs）主要富集于萜烯合成。在红色主根逐渐形成的过程中，参与丹参酮合成的大部分基因被上调。植物激素信号转导和ABC转运途径广泛参与丹参主根发育。根据共表达模式筛选出5个可能参与或调控丹参酮合成的候选基因。此外，预计光合氧化铁还蛋白（FD）、细胞色素P450还原酶（CPR）和CCAAT结合转录因子（CBF）与已知的下游必需酶基因直接相互作用。上述结果为分析丹参酮的初始合成及其调控机制提供了必要的依据。
Tanshinones are the bioactive constituents of Danshen (Salvia miltiorrhiza Bunge), which is used in Traditional Chinese Medicine to treat cardiovascular and other diseases, and they synthesize and accumulate in the root periderm of S. miltiorrhiza. However, there is no relevant report on the initial stage of tanshinone synthesis, as well as the root structure and gene expression characteristics. The present study aims to provide new insights into how these bioactive principles begin to synthesize by characterizing possible differences in their biosynthesis and accumulation during early root development from both spatial and temporal aspects. The morphological characteristics and the content of tanshinones in roots of S. miltiorrhiza were investigated in detail by monitoring the seedlings within 65 days after germination (DAGs). The ONT transcriptome sequencing was applied to investigate gene expression patterns. The periderm of the S. miltiorrhiza storage taproot initially synthesized tanshinone on about 30 DAGs. Three critical stages of tanshinone synthesis were preliminarily determined: preparation, the initial synthesis, and the continuous rapid synthesis. The difference of taproots in the first two stages was the smallest, and the differentially expressed genes (DEGs) were mainly enriched in terpene synthesis. Most genes involved in tanshinone synthesis were up regulated during the gradual formation of the red taproot. Plant hormone signal transduction and ABC transport pathways were widely involved in S. miltiorrhiza taproot development. Five candidate genes that may participate in or regulate tanshinone synthesis were screened according to the co-expression pattern. Moreover, photosynthetic ferredoxin (FD), cytochrome P450 reductase (CPR), and CCAAT binding transcription factor (CBF) were predicted to interact with the known downstream essential enzyme genes directly. The above results provide a necessary basis for analyzing the initial synthesis and regulation mechanism of Tanshinones.
登录
查看更多内容
影响因子：
2.7
作者：
Cheng, Qiqing;Su, Ping;Huang, Luqi
通讯作者：
Huang, Luqi
丹参SmGGPPs启动子的分子克隆、特性及其表达模式
影响因子：
2.8
作者：
Hua, Wenping;Song, Jie;Wang, Zhezhi
通讯作者：
Wang, Zhezhi
影响因子：
11.6
作者：
Jasinski, M;Stukkens, Y;Boutry, M
通讯作者：
Boutry, M
影响因子：
5.6
作者：
Li, Wenrui;Bai, Zhenqing;Liang, Zongsuo
通讯作者：
Liang, Zongsuo
丹参毛状根培养物中诱导子诱导丹参酮积累的分子机制
影响因子：
2.6
作者：
Kai, Guoyin;Liao, Pan;Zhang, Lin
通讯作者：
Zhang, Lin