Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia.
Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia.
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DOI：
10.1016/j.regpep.2009.02.002
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发表时间：
2009-04-10
影响因子：
--
通讯作者：
Saavedra, Juan M.
中科院分区：
文献类型：
--
作者：
Imboden, Hans;Patil, Jaspal;Nussberger, Juerg;Nicoud, Francoise;Hess, Benno;Ahmed, Nermin;Schaffner, Thomas;Wellner, Maren;Mueller, Dominik;Inagami, Tadashi;Senbonmatsu, Takaaki;Pavel, Jaroslav;Saavedra, Juan M.
为了阐明血管紧张素 II (Ang II) 在感觉系统尤其是三叉神经节中的作用，我们研究了血管紧张素原 (Ang-N)、肾素、血管紧张素转换酶 (ACE) 和组织蛋白酶 D-mRNA 的表达，以及大鼠和人三叉神经节中 Ang II 和 P 物质的存在。通过定量实时 PCR 测定，大鼠三叉神经节表达大量 Ang-N- 和 ACE mRNA。在大鼠样本中无法追踪到肾素 mRNA。在大鼠三叉神经节中检测到组织蛋白酶 D，表明可能存在血管紧张素 I 形成的肾素替代途径。大鼠三叉神经节的原位杂交揭示了许多神经元细胞质中表达 Ang-N mRNA。通过使用免疫细胞化学，对大鼠和人三叉神经节中的许多神经元及其突起进行了 Ang II 染色。原位杂交后免疫细胞化学显示，在大鼠三叉神经节中，一些（但不是所有）Ang-N mRNA 阳性神经元被标记为 Ang II。在一些神经元中，发现 P 物质与 Ang II 共定位。通过放射免疫测定法对来自大鼠三叉神经节的血管紧张素进行定量，无论是否经过高效液相色谱法预先分离。免疫反应性血管紧张素 II (ir-Ang II) 始终存在，并且真正的 Ang II (1-8) 八肽及其专门测量的代谢物的总和被发现可以解释这一点。神经元组织中 ir-Ang II 的放射免疫学和免疫细胞化学证据与 Ang II 作为神经递质相容。总之，这些结果表明Ang II可以在大鼠三叉神经节的神经元中局部产生。神经元 Ang II 与 P 物质在三叉神经节神经元中的定位和共定位可能是 Ang II 在伤害感受和偏头痛病理调节中参与和发挥作用的基础。
To clarify the role of Angiotensin II (Ang II) in the sensory system and especially in the trigeminal ganglia, we studied the expression of angiotensinogen (Ang-N)-, renin-, angiotensin converting enzyme (ACE)- and cathepsin D-mRNA, and the presence of Ang II and substance P in the rat and human trigeminal ganglia. The rat trigeminal ganglia expressed substantial amounts of Ang-N- and ACE mRNA as determined by quantitative real time PCR. Renin mRNA was untraceable in rat samples. Cathepsin D was detected in the rat trigeminal ganglia indicating the possibility of existence of pathways alternative to renin for Ang I formation. In situ hybridization in rat trigeminal ganglia revealed expression of Ang-N mRNA in the cytoplasm of numerous neurons. By using immunocytochemistry, a number of neurons and their processes in both the rat and human trigeminal ganglia were stained for Ang II. Post in situ hybridization immunocytochemistry reveals that in the rat trigeminal ganglia some, but not all Ang-N mRNA-positive neurons marked for Ang II. In some neurons Substance P was found colocalized with Ang II. Angiotensins from rat trigeminal ganglia were quantitated by radioimmunoassay with and without prior separation by high performance liquid chromatography. Immunoreactive angiotensin II (ir-Ang II) was consistently present and the sum of true Ang II (1-8) octapeptide and its specifically measured metabolites were found to account for it. Radioimmunological and immunocytochemical evidence of ir-Ang II in neuronal tissue is compatible with Ang II as a neurotransmitter. In conclusion, these results suggest that Ang II could be produced locally in the neurons of rat trigeminal ganglia. The localization and colocalization of neuronal Ang II with Substance P in the trigeminal ganglia neurons may be the basis for a participation and function of Ang II in the regulation of nociception and migraine pathology.
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影响因子：
5
作者：
Charles, JA;Jotkowitz, S;Byrd, LH
通讯作者：
Byrd, LH
影响因子：
12.3
作者：
CHAI, CY;HELLMANN, W;GANTEN, D
通讯作者：
GANTEN, D
影响因子：
2.1
作者：
DOSTROVSKY, JO;DAVIS, KD;KAWAKITA, K
通讯作者：
KAWAKITA, K
影响因子：
--
作者：
Frei, N;Weissenberger, J;Imboden, H
通讯作者：
Imboden, H
DOI：
10.1016/s0169-328x(97)00339-2
发表时间：
1998-03-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Baltatu, O;Lippoldt, A;Bader, M
通讯作者：
Bader, M