Targeted diversity generation by intraterrestrial archaea and archaeal viruses.
Targeted diversity generation by intraterrestrial archaea and archaeal viruses.
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DOI：
10.1038/ncomms7585
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发表时间：
2015-03-23
影响因子：
16.6
通讯作者：
Valentine, David L.
中科院分区：
文献类型：
--
作者：
Paul, Blair G.;Bagby, Sarah C.;Czornyj, Elizabeth;Arambula, Diego;Handa, Sumit;Sczyrba, Alexander;Ghosh, Partho;Miller, Jeff F.;Valentine, David L.
在微生物、它们的寄生虫和它们的邻居之间的进化军备竞赛中，蛋白质快速多样化的能力是一种有力的武器。多样性生成逆转录元件（DGR）使用诱变逆转录和逆转录归巢来产生靶基因的无数变体。最初在病原体中发现，这些逆转录因子已在细菌及其病毒中发现，但从未在古细菌中发现。在这里，我们报告发现完整的DGRs在两个不同的地内古菌系统：一种新的病毒，似乎感染古菌在海洋地下，和，分别，两个未培养的nanoarchaea从陆地地下。病毒DGR系统靶向推定的尾纤维配体结合结构域，可能产生>1018个蛋白变体。两个单细胞纳米古细菌基因组各自具有≥4个不同的DGR。在预期的低全基因组突变率的背景下，这些结果证明了先前未被怀疑的潜在的快速，有针对性的序列多样化的地内古生菌及其病毒。 多样性生成逆转录元件（DGR）是在细菌及其病毒的靶基因内引入序列变异的遗传元件。在这里，Paul等人报告了分别在海洋和陆地地下环境中的古细菌病毒和两种古细菌中发现DGRs。
In the evolutionary arms race between microbes, their parasites, and their neighbours, the capacity for rapid protein diversification is a potent weapon. Diversity-generating retroelements (DGRs) use mutagenic reverse transcription and retrohoming to generate myriad variants of a target gene. Originally discovered in pathogens, these retroelements have been identified in bacteria and their viruses, but never in archaea. Here we report the discovery of intact DGRs in two distinct intraterrestrial archaeal systems: a novel virus that appears to infect archaea in the marine subsurface, and, separately, two uncultivated nanoarchaea from the terrestrial subsurface. The viral DGR system targets putative tail fibre ligand-binding domains, potentially generating >1018 protein variants. The two single-cell nanoarchaeal genomes each possess ≥4 distinct DGRs. Against an expected background of low genome-wide mutation rates, these results demonstrate a previously unsuspected potential for rapid, targeted sequence diversification in intraterrestrial archaea and their viruses. Diversity-generating retroelements (DGRs) are genetic elements that introduce sequence variation within target genes in bacteria and their viruses. Here, Paul et al. report the discovery of DGRs in an archaeal virus and in two archaea from marine and terrestrial subsurface environments, respectively.
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影响因子：
3.4
作者：
Dwivedi B;Xue B;Lundin D;Edwards RA;Breitbart M
通讯作者：
Breitbart M
影响因子：
5.1
作者：
Lewin, Anna;Johansen, Jostein;Valla, Svein
通讯作者：
Valla, Svein
影响因子：
64.8
作者：
Huber, H;Hohn, MJ;Stetter, KO
通讯作者：
Stetter, KO
影响因子：
3.7
作者：
Hurwitz BL;Sullivan MB
通讯作者：
Sullivan MB
DOI：
10.1073/pnas.1203849109
发表时间：
2012-10-02
影响因子：
11.1
作者：
Kallmeyer, Jens;Pockalny, Robert;D'Hondt, Steven
通讯作者：
D'Hondt, Steven