Electrical Guidance of Human Stem Cells in the Rat Brain.
Electrical Guidance of Human Stem Cells in the Rat Brain.
复制标题
大鼠大脑中人类干细胞的电引导
DOI：
10.1016/j.stemcr.2017.05.035
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发表时间：
2017-07-11
影响因子：
5.9
通讯作者：
Zhao M
中科院分区：
文献类型：
--
作者：
Feng JF;Liu J;Zhang L;Jiang JY;Russell M;Lyeth BG;Nolta JA;Zhao M
神经干细胞在成人脑中的有限迁移是使用干细胞疗法治疗脑疾病和损伤的障碍。在这里，我们报告了一种动员和引导干细胞在体内大脑中迁移的策略。我们开发了一种安全的刺激模式，在大脑中传递定向电流。跟踪表达GFP的细胞证明了人类神经干细胞的迁移的电动员和指导，即使是针对喙部迁移流中共存的内在线索。在刺激后3周和4个月，在由刺激电流引导的区域中观察移植的细胞，并观察分化的迹象。因此，电刺激可能提供一种促进脑干细胞治疗的潜在方法。开发了一种在体内向大脑输送电流的技术和装置实现了向大脑稳定输送电流，并考虑了监测和安全性问题显示出移植的人NSC在活脑中迁移的有效引导显示出被引导的hNSC的运动性、存活和分化增强在这篇文章中，Zhao及其同事报告了一种新的技术，该技术提供定向电流，可以动员和引导人类神经干细胞在体内通过大脑，证明了一种有效和安全的方法来促进干细胞治疗，对广泛的脑部疾病具有重要意义。
Limited migration of neural stem cells in adult brain is a roadblock for the use of stem cell therapies to treat brain diseases and injuries. Here, we report a strategy that mobilizes and guides migration of stem cells in the brain in vivo. We developed a safe stimulation paradigm to deliver directional currents in the brain. Tracking cells expressing GFP demonstrated electrical mobilization and guidance of migration of human neural stem cells, even against co-existing intrinsic cues in the rostral migration stream. Transplanted cells were observed at 3 weeks and 4 months after stimulation in areas guided by the stimulation currents, and with indications of differentiation. Electrical stimulation thus may provide a potential approach to facilitate brain stem cell therapies. Developed a technology and device delivering electric current to the brain in vivo Achieved stable delivery of currents to brain with monitoring and safety concerns Exhibited effective guidance of migration of transplanted human NSCs in live brain Demonstrated enhanced motility, survival, and differentiation of the guided hNSCs In this article, Zhao and colleagues report a novel technology delivering directional electric currents which mobilizes and guides human neural stem cells through the brain in vivo, demonstrating an effective and safe approach to facilitate stem cell therapy, with significant implications for a wide range of brain diseases.
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影响因子：
5.3
作者：
Meng, Xiaoting;Arocena, Miguel;Penninger, Josef;Gage, Fred H.;Zhao, Min;Song, Bing
通讯作者：
Song, Bing
影响因子：
4.2
作者：
HAMM, RJ;PIKE, BR;JENKINS, LW
通讯作者：
JENKINS, LW
影响因子：
16.2
作者：
LUSKIN, MB
通讯作者：
LUSKIN, MB
影响因子：
56.9
作者：
Famulski, Jakub K.;Trivedi, Niraj;Solecki, David J.
通讯作者：
Solecki, David J.
影响因子：
3
作者：
Metz, GA;Whishaw, IQ
通讯作者：
Whishaw, IQ