Pan-Genome Analysis of Laribacter hongkongensis: Virulence Gene Profiles, Carbohydrate-Active Enzyme Prediction, and Antimicrobial Resistance Characterization.
Pan-Genome Analysis of Laribacter hongkongensis: Virulence Gene Profiles, Carbohydrate-Active Enzyme Prediction, and Antimicrobial Resistance Characterization.
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DOI：
10.3389/fmicb.2022.862776
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
香港海鸥菌是一种新发现的食源性致病菌，可引起社区获得性胃肠炎和旅行者腹泻。然而，L. hongkonggensis尚未被正确理解。共采集到45株水生动物相关L.为全面了解这一食源性新致病菌，对从青蛙和草鱼肠道标本中分离的香港分离株进行了全基因组测序，沿着4株人类临床分离株的基因组数据，并对其毒力基因、碳水化合物活性酶和耐药性决定因子进行了分析。从系统发育树推断，人类临床菌株与来自青蛙的一些菌株在遗传上更接近。不同来源菌株的毒力基因和碳水化合物活性酶的分布具有不同的模式，反映了它们对不同宿主环境的适应性，表明它们感染人类的潜力不同。检测32个耐药性基因，并对氨基糖苷类、氯霉素、甲氧苄啶、磺胺类等18种临床常用抗生素进行药敏试验，评价临床用药的可利用性。对利福平、头孢唑啉、头孢他啶、氨苄西林、头孢曲松的耐药率较高，对四环素、复方新诺明、环丙沙星、左氧氟沙星的耐药率在近半数蛙源菌株中呈聚集性分布，提示蛙源菌株耐药情况较为严重，临床治疗对L.感染香港人应加倍小心。
Laribacter hongkongensis is a new emerging foodborne pathogen that causes community-acquired gastroenteritis and traveler’s diarrhea. However, the genetic features of L. hongkongensis have not yet been properly understood. A total of 45 aquatic animal-associated L. hongkongensis strains isolated from intestinal specimens of frogs and grass carps were subjected to whole-genome sequencing (WGS), along with the genome data of 4 reported human clinical strains, the analysis of virulence genes, carbohydrate-active enzymes, and antimicrobial resistance (AMR) determinants were carried out for comprehensively understanding of this new foodborne pathogen. Human clinical strains were genetically more related to some strains from frogs inferred from phylogenetic trees. The distribution of virulence genes and carbohydrate-active enzymes exhibited different patterns among strains of different sources, reflecting their adaption to different host environments and indicating different potentials to infect humans. Thirty-two AMR genes were detected, susceptibility to 18 clinical used antibiotics including aminoglycoside, chloramphenicol, trimethoprim, and sulfa was checked to evaluate the availability of clinical medicines. Resistance to Rifampicin, Cefazolin, ceftazidime, Ampicillin, and ceftriaxone is prevalent in most strains, resistance to tetracycline, trimethoprim-sulfamethoxazole, ciprofloxacin, and levofloxacin are aggregated in nearly half of frog-derived strains, suggesting that drug resistance of frog-derived strains is more serious, and clinical treatment for L. hongkongensis infection should be more cautious.
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影响因子：
14.9
作者：
Letunic I;Bork P
通讯作者：
Bork P
影响因子：
3.7
作者：
Kong HK;Law HW;Liu X;Law CO;Pan Q;Gao L;Xiong L;Lau SK;Woo PC;Lau TC
通讯作者：
Lau TC
影响因子：
5.8
作者：
Hernandez, David;Tewhey, Ryan;Schrenzel, Jacques
通讯作者：
Schrenzel, Jacques
影响因子：
9.8
作者：
Greay, Telleasha L.;Gofton, Alexander W.;Ryan, Una M.
通讯作者：
Ryan, Una M.
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T