Variations in culturable bacterial communities and biochemical properties in the foreland of the retreating Tianshan No. 1 glacier.
Variations in culturable bacterial communities and biochemical properties in the foreland of the retreating Tianshan No. 1 glacier.
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天山一号冰川消退前陆可培养细菌群落及生化特性的变化
DOI：
10.1016/j.bjm.2018.01.001
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Wu X;Zhang G;Zhang W;Liu G;Chen T;Wang Y;Long H;Tai X;Zhang B;Li Z
随着冰川的消退，贫瘠的地区暴露出来，这些贫瘠的地区是研究微生物演替的理想地点。在这项研究中，我们对天山冰川消退早期演替土壤的可培养细菌群落和生化参数进行了表征。在4℃和25℃时，可培养细菌总数分别为2.19亿×105cfu/g−1dw和9.33亿×106cfu/g−1dw。土壤中可培养细菌的数量在25℃时增加，在4℃时减少。冰川前陆的总有机碳、总氮含量和酶活性相对较低。在25℃下分离到的可培养细菌数量与土壤总有机碳、总氮以及土壤尿素酶、蛋白酶、多酚氧化酶、蔗糖酶、过氧化氢酶和脱氢酶活性呈显著正相关。通过扩增核糖体DNA限制性内切酶分析，从冰川前陆土壤中获得了358个分离物，聚为35个类群。这些类群隶属于20个属，隶属于6个分类群，即α-蛋白细菌、β-蛋白细菌、伽马蛋白细菌、放线杆菌、类杆菌和热变形球菌，所有样品中都以放线杆菌和变形杆菌占优势。冗余度分析表明，细菌演替分为早期(10a)、中期(25-74a)和晚期(100-130a)三个阶段，可培养细菌总数主要受土壤酶活性的影响，表明微生物演替与前陆土壤年龄有关。
As a glacier retreats, barren areas are exposed, and these barren areas are ideal sites to study microbial succession. In this study, we characterized the soil culturable bacterial communities and biochemical parameters of early successional soils from a receding glacier in the Tianshan Mountains. The total number of culturable bacteria ranged from 2.19 × 105 to 1.30 × 106 CFU g−1 dw and from 9.33 × 105 to 2.53 × 106 CFU g−1 dw at 4 °C and 25 °C, respectively. The number of culturable bacteria in the soil increased at 25 °C but decreased at 4 °C along the chronosequence. The total organic carbon, total nitrogen content, and enzymatic activity were relatively low in the glacier foreland. The number of culturable bacteria isolated at 25 °C was significantly positively correlated with the TOC and TN as well as the soil urease, protease, polyphenoloxidase, sucrase, catalase, and dehydrogenase activities. We obtained 358 isolates from the glacier foreland soils that clustered into 35 groups using amplified ribosomal DNA restriction analysis. These groups are affiliated with 20 genera that belong to six taxa, namely, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Bacteroides, and Deinococcus-Thermus, with a predominance of members of Actinobacteria and Proteobacteria in all of the samples. A redundancy analysis showed that the bacterial succession was divided into three periods, an early stage (10a), a middle stage (25–74a), and a late stage (100–130a), with the total number of culturable bacteria mainly being affected by the soil enzymatic activity, suggesting that the microbial succession correlated with the soil age along the foreland.
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影响因子：
9.7
作者：
Saiya-Cork, KR;Sinsabaugh, RL;Zak, DR
通讯作者：
Zak, DR
影响因子：
9.7
作者：
SCHINNER, F;VONMERSI, W
通讯作者：
VONMERSI, W
影响因子：
2.6
作者：
Bai, Yu;Yang, Daqun;An, Lizhe
通讯作者：
An, Lizhe
影响因子：
4.8
作者：
Li, Junjian;Zhou, Xiaomei;He, Jizheng
通讯作者：
He, Jizheng
影响因子：
--
作者：
NANNIPIERI, P;PEDRAZZINI, F;PIOVANELLI, C
通讯作者：
PIOVANELLI, C