Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses
Expression of a grape bZIP transcription factor, VqbZIP39, in transgenic Arabidopsis thaliana confers tolerance of multiple abiotic stresses
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转基因拟南芥中葡萄 bZIP 转录因子 VqbZIP39 的表达赋予其对多种非生物胁迫的耐受性
DOI：
10.1007/s11240-016-0969-6
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发表时间：
2016-03
影响因子：
3
通讯作者：
Wang, Xiping
中科院分区：
文献类型：
--
作者：
Guo, Rongrong;Zhang, Hongjing;Cai, Junshe;Wang, Xiping
碱性区/亮氨酸拉链（bZIP）转录因子是已知的在响应非生物胁迫中发挥关键作用。本研究从葡萄（葡萄属毛葡萄）中分离出bZIP基因（VqbZIP 39），并在花椰菜花叶病毒35 S启动子的控制下在拟南芥中组成型表达。转基因拟南芥在种子萌发期、幼苗期和成熟期表现出较强的耐盐性和耐旱性。分析了与胁迫反应相关的各种生理参数以进一步了解VqbZIP 39的作用，并且发现渗透胁迫对转基因幼苗造成的损害比相应的野生型植物小。这与VqbZIP 39的组成性过表达导致的内源阿坝含量的增加以及与干旱、高盐和氧化胁迫耐受性相关的胁迫诱导靶基因的上调表达相关。我们的结果表明，VqbZIP 39在A.拟南芥可能通过阿坝信号途径增强对多种非生物胁迫的耐受性，因此在葡萄对非生物胁迫的响应中可能具有类似的功能。
The basic region/leucine zipper (bZIP) transcription factors are known to play key roles in response to abiotic stress. In this study, a bZIP gene (VqbZIP39) was isolated from grape (Vitis quinquangularis) and constitutively expressed in Arabidopsis under control of the cauliflower mosaic virus 35S promoter. The transgenic Arabidopsis thaliana plants showed enhance salt and drought stress tolerance during seed germination and in the seedling and mature plant stages. Various physiological parameters related to stress responses were analyzed to gain further insight into the role of VqbZIP39 and it was found that osmotic stress caused less damage to the transgenic seedlings than to the corresponding wild type plants. This correlated with an increase in endogenous ABA content as a consequence of the constitutive over-expression of VqbZIP39, and the up-regulated expression of stress-inducible target genes associated with tolerance of drought, high-salt, and oxidative stresses. Our results suggest that the expression of VqbZIP39 in A. thaliana likely enhances the tolerance to multiple abiotic stresses through the ABA signaling pathway, and may therefore have a similar function in the response to abiotic stresses in grape.
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影响因子：
5.3
作者：
Liu GT;Wang JF;Cramer G;Dai ZW;Duan W;Xu HG;Wu BH;Fan PG;Wang LJ;Li SH
通讯作者：
Li SH
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影响因子：
4.3
作者：
Liu, Citao;Wu, Yanbin;Wang, Xiping
通讯作者：
Wang, Xiping
影响因子：
7.3
作者：
Kotchoni, Simeon O.;Kuhns, Christine;Bartels, Dorothea
通讯作者：
Bartels, Dorothea
DOI：
10.1007/s11240-012-0142-9
发表时间：
2012-03
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
Hui Zhang;Yaxin Liu;Yuan Xu;S. Chapman;A. Love;Tao Xia
通讯作者：
Hui Zhang;Yaxin Liu;Yuan Xu;S. Chapman;A. Love;Tao Xia
影响因子：
6.9
作者：
Fryer, MJ;Oxborough, K;Baker, NR
通讯作者：
Baker, NR