Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress.
Transcriptomic analysis of grape (Vitis vinifera L.) leaves during and after recovery from heat stress.
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葡萄（葡萄属vinifera L.）从热胁迫中恢复期间和之后的叶子。
DOI：
10.1186/1471-2229-12-174
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发表时间：
2012-09-28
影响因子：
5.3
通讯作者：
Li SH
中科院分区：
文献类型：
--
作者：
Liu GT;Wang JF;Cramer G;Dai ZW;Duan W;Xu HG;Wu BH;Fan PG;Wang LJ;Li SH
葡萄是世界上主要的水果作物。高温胁迫可显著降低葡萄产量和品质。在分子水平上对热应激和随后的恢复的反应的变化知之甚少。为了阐明热胁迫和随后恢复对葡萄叶片中代表经典热胁迫反应和耐热机制的基因表达的影响，以葡萄（葡萄属vinifera L.）使用Affyscore葡萄基因组寡核苷酸微阵列（15，700个转录物）对叶片进行定量，然后对一些转录物谱进行定量实时PCR验证。我们发现，约8%的总探针集响应于热胁迫和/或随后的恢复在葡萄叶片。热胁迫和恢复反应的特点是不同的转录变化。热应激调控基因的数量几乎是恢复调控基因数量的两倍。本研究中鉴定的应答基因属于大量重要性状和生物学途径，包括细胞拯救（即，抗氧化酶），蛋白质命运（即，热休克蛋白），初级和次级代谢，转录因子，信号转导和发育。我们已经确定了一些共同的基因和热休克因子（HSFs）的差异调制热应激和恢复。大多数HSP基因上调热应激，但下调的恢复。另一方面，一些特定的HSP基因或HSFs对热应激或恢复具有独特的反应。热胁迫对葡萄的影响和恢复与胁迫相关基因、转录因子和代谢等多个过程和机制有关。热应激和恢复引起共同的上调或下调基因以及独特的反应基因组。此外，一些基因的调控方向相反，热应激和恢复。结果表明，热休克蛋白，特别是小热休克蛋白，抗氧化酶（即，抗坏血酸过氧化物酶）和半乳糖苷醇合成酶可能对葡萄的耐热性起重要作用。HSF 30可能是热应激和恢复的关键调节因子，而HSF 7和HSF 1可能仅特异于恢复。本研究中热胁迫或恢复反应基因的鉴定为葡萄叶片耐热性的分子基础提供了新的见解。
Grapes are a major fruit crop around the world. Heat stress can significantly reduce grape yield and quality. Changes at the molecular level in response to heat stress and subsequent recovery are poorly understood. To elucidate the effect of heat stress and subsequent recovery on expression of genes by grape leaves representing the classic heat stress response and thermotolerance mechanisms, transcript abundance of grape (Vitis vinifera L.) leaves was quantified using the Affymetrix Grape Genome oligonucleotide microarray (15,700 transcripts), followed by quantitative Real-Time PCR validation for some transcript profiles. We found that about 8% of the total probe sets were responsive to heat stress and/or to subsequent recovery in grape leaves. The heat stress and recovery responses were characterized by different transcriptional changes. The number of heat stress-regulated genes was almost twice the number of recovery-regulated genes. The responsive genes identified in this study belong to a large number of important traits and biological pathways, including cell rescue (i.e., antioxidant enzymes), protein fate (i.e., HSPs), primary and secondary metabolism, transcription factors, signal transduction, and development. We have identified some common genes and heat shock factors (HSFs) that were modulated differentially by heat stress and recovery. Most HSP genes were upregulated by heat stress but were downregulated by the recovery. On the other hand, some specific HSP genes or HSFs were uniquely responsive to heat stress or recovery. The effect of heat stress and recovery on grape appears to be associated with multiple processes and mechanisms including stress-related genes, transcription factors, and metabolism. Heat stress and recovery elicited common up- or downregulated genes as well as unique sets of responsive genes. Moreover, some genes were regulated in opposite directions by heat stress and recovery. The results indicated HSPs, especially small HSPs, antioxidant enzymes (i.e., ascorbate peroxidase), and galactinol synthase may be important to thermotolerance of grape. HSF30 may be a key regulator for heat stress and recovery, while HSF7 and HSF1 may only be specific to recovery. The identification of heat stress or recovery responsive genes in this study provides novel insights into the molecular basis for heat tolerance in grape leaves.
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影响因子：
6.9
作者：
Frank G;Pressman E;Ophir R;Althan L;Shaked R;Freedman M;Shen S;Firon N
通讯作者：
Firon N
影响因子：
5.3
作者：
Jarosová J;Kundu JK
通讯作者：
Kundu JK
影响因子：
6.1
作者：
Deluc LG;Decendit A;Papastamoulis Y;Mérillon JM;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K