Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana

Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana
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从苎麻 (Boehmeria nivea L. Gaudich) 中分离两个新型启动子及其在拟南芥中的功能表征

DOI:
10.1007/s11240-018-01528-9
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发表时间:
2018-12
期刊:
Plant Cell, Tissue and Organ Culture
影响因子:
--
通讯作者:
Peng Dingxiang
Peng Dingxiang
中科院分区:
其他
文献类型:
--
作者:
Guo Pingan;Zheng Yancheng;Chen Jie;Wang Bo;Liu Lijun;Feng Enying;Peng Dingxiang

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组织特异性启动子或诱导型启动子的开发对植物基因工程具有重要意义。在本研究中,我们从苎麻中分离到两个新的启动子(命名为BnGDSPO和BnSTMpro),并对它们在拟南芥中的功能进行了分析。结果表明,BnGDSpro及其5‘端截短片段驱动GUS主要在茎分生组织中表达,而BnSTMpro及其5’端截短片段在拟南芥幼苗中具有顶端分生组织特异性,在花和角果中具有根瘤特异性。BnSTMpro1233~− 914区和− 425~0区分别是根尖专一性和根结专一性的区域。特别是CuSO4处理表明,BnGDS和BnSTMpro都是铜诱导启动子。我们的研究结果表明,BnGDSpro和BnSTMpro及其截短片段在植物遗传转化中具有潜在的应用前景。
The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named BnGDSpro and BnSTMpro) from ramie and analyzed their functions in Arabidopsis. The results show that BnGDSpro and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while BnSTMpro and its 5′-truncated fragments have apical meristem specificity in Arabidopsis seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of BnSTMpro are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO4 treatment reveals that both BnGDSpro and BnSTMpro are copper-inducible promoters. Our findings suggest that BnGDSpro and BnSTMpro and their truncated fragments have potential applications in plant genetic transformation.
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