Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana
Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana
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从苎麻 (Boehmeria nivea L. Gaudich) 中分离两个新型启动子及其在拟南芥中的功能表征
DOI:
10.1007/s11240-018-01528-9
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发表时间:
2018-12
期刊:
影响因子:
--
通讯作者:
Peng Dingxiang
中科院分区:
文献类型:
--
作者:
Guo Pingan;Zheng Yancheng;Chen Jie;Wang Bo;Liu Lijun;Feng Enying;Peng Dingxiang
The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named BnGDSpro and BnSTMpro) from ramie and analyzed their functions in Arabidopsis. The results show that BnGDSpro and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while BnSTMpro and its 5′-truncated fragments have apical meristem specificity in Arabidopsis seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of BnSTMpro are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO4 treatment reveals that both BnGDSpro and BnSTMpro are copper-inducible promoters. Our findings suggest that BnGDSpro and BnSTMpro and their truncated fragments have potential applications in plant genetic transformation.
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影响因子:
9.5
作者:
Katoh K;Rozewicki J;Yamada KD
通讯作者:
Yamada KD
影响因子:
5.2
作者:
Li, Yuanya;Liu, Shaojun;Wu, Ping
通讯作者:
Wu, Ping
影响因子:
5.6
作者:
An X;Chen J;Zhang J;Liao Y;Dai L;Wang B;Liu L;Peng D
通讯作者:
Peng D
DOI:
10.1002/j.1460-2075.1987.tb02730.x
发表时间:
1987-12
期刊:
The EMBO Journal
影响因子:
--
作者:
R. Jefferson;T. Kavanagh;M. Bevan
通讯作者:
R. Jefferson;T. Kavanagh;M. Bevan
DOI:
--
发表时间:
--
期刊:
--
影响因子:
--
作者:
K. Livak;Thomas D. Schmittgen
通讯作者:
K. Livak;Thomas D. Schmittgen