Molecular characterization of a novel cryptic virus infecting pigeonpea plants.
Molecular characterization of a novel cryptic virus infecting pigeonpea plants.
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DOI：
10.1371/journal.pone.0181829
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Hallan V
中科院分区：
文献类型：
--
作者：
Kumar S;Subbarao BL;Kumari R;Hallan V
该病毒属的一个新成员含有3个dsRNAs，大小分别为1.71、1.49和1.43kb。从无症状木豆[Cajanus cajan(L.)访问数/每百万人：Reach for[埃拉·坎杜鲁。这一新病毒基因组为4.64kb，暂定名为Arhar隐蔽病毒-1(ARCV-1)。对基因组RNA进行扩增，并用不依赖序列的单引物扩增对其进行鉴定。DsRNA共有一个高度保守的16个核苷酸5‘非编码区(5’-GATAATGATCCAAGGA-3‘)。最大的dsRNA(dsRNA-1)被鉴定为病毒RNA依赖的RNA聚合酶(复制酶)，预测编码55.34 kDa的蛋白(P1)。另外两个较小的dsRNA(dsRNA-2和dsRNA-3)分别编码38.50 kDa(P2)和38.51 kDa(P3)的衣壳蛋白。系统进化树分析表明，ArCV-1与花冠潜伏病毒、多花月季潜伏病毒和玫瑰潜伏病毒形成了一个分支，表明ArCV-1可能是DelapartitiVirus的一个新成员。ArCV-1的3Dpol结构揭示了几个有趣的特征。3Dpol的全长在结构上与Caliciviridae和PicorNaviridae家族的成员有相似之处。此外，在同一植物组织中还发现了与ArCV-1基因组无关的第四个dsRNA分子(dsRNA-2A)。DsRNA-2A(1.6kb)编码一个蛋白质(P4)，预测大小为44.5 kDa。P4与几种隐蔽病毒的外壳蛋白基因具有相似性，特别是包括萝卜隐蔽病毒-3在内的两部分隐蔽病毒。这是首次在木豆植物中发现一种隐蔽病毒的报告。
A new member of the genus Deltapartitivirus was identified containing three dsRNAs with an estimated size of 1.71, 1.49 and 1.43 kb. The dsRNAs were extracted from symptomless pigeonpea [Cajanus cajan (L.) Millspaugh] plants cv. Erra Kandulu. This new virus with 4.64 kb genome was tentatively named Arhar cryptic virus-1 (ArCV-1). The genomic RNAs were amplified and characterized by sequence independent single primer amplification. The dsRNAs shared a highly conserved 16 nt 5’ non-coding region (5’-GATAATGATCCAAGGA-3’). The largest dsRNA (dsRNA-1) was identified as the viral RNA dependent RNA polymerase (replicase), predicted to encode a putative 55.34 kDa protein (P1). The two other smaller dsRNAs (dsRNA-2 and dsRNA-3) predicted to encode for putative capsid proteins of 38.50kDa (P2) and 38.51kDa (P3), respectively. Phylogenetic analysis indicated that ArCV-1 formed a clade together with Fragaria chiloensis cryptic virus, Rosa multiflora cryptic virus and Rose cryptic virus-1, indicating that ArCV-1 could be a new member of the genus Deltapartitivirus. ArCV-1 3Dpol structure revealed several interesting features. The 3Dpol in its full-length shares structural similarities with members of the family Caliciviridaeand family Picornaviridae. In addition, fourth dsRNA molecule (dsRNA-2A), not related to ArCV-1 genome, was found in the same plant tissue. The dsRNA-2A (1.6 kb) encodes a protein (P4), with a predicted size of 44.5 kDa. P4 shares similarity with coat protein genes of several cryptic viruses, in particular the bipartite cryptic viruses including Raphanus sativus cryptic virus-3. This is the first report of occurrence of a cryptic virus in pigeonpea plants.
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