Subcellular distribution and chemical forms of cadmium in a dark septate endophyte (DSE), Exophiala pisciphila
Subcellular distribution and chemical forms of cadmium in a dark septate endophyte (DSE), Exophiala pisciphila
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深色有隔内生菌 (DSE) Exophiala pisciphila 中镉的亚细胞分布和化学形态
DOI：
10.1007/s11356-015-5012-7
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发表时间：
2015-07
影响因子：
5.8
通讯作者：
Zhao Zhiwei
中科院分区：
文献类型：
--
作者：
Zhan Fangdong;He Yongmei;Li Yuan;Li Tao;Yang Yun-Ya;Toor Gurpal S.;Zhao Zhiwei
通过研究嗜鱼外瓶霉菌丝体中镉的亚细胞分布、化学形态和吸附基团，探讨其对镉的耐受机制。我们种植了E。随着Cd浓度的增加（0、25、50、100、200和400 mg L（-1）），pisciphila在液体培养基中的生长受到抑制。培养基中Cd含量的增加导致E.鱼友亚细胞分布表明，81%至97%的镉与细胞壁。2%乙酸对Cd的萃取量和比例最大（45-86%），且萃取量与乙酸浓度有关，表明Cd-磷酸盐络合物是大肠杆菌中Cd的主要化学形态。鱼友用扫描电镜-能谱仪（SEM-EDS）观察到菌丝体表面有大量的磷酸盐和镉的分布。通过透射电子显微镜-能量色散X射线能谱（TEM-EDX）观察到与菌丝体相关的沉淀物含有镉。傅里叶变换红外光谱（FTIR）确定羟基，胺，羧基，磷酸基团负责结合镉。我们认为，Cd与细胞壁结合，并与磷酸盐结合，可能是导致E. pisciphila为Cd.
Our objective was to understand the cadmium (Cd) tolerance mechanisms by investigating the subcellular distribution, chemical forms of Cd and adsorptive groups in the mycelia of Exophiala pisciphila. We grew E. pisciphila in the liquid media with increasing Cd concentrations (0, 25, 50, 100, 200, and 400 mg L(-1)). Increased Cd in the media caused a proportional increase in the Cd uptake by E. pisciphila. Subcellular distribution indicated that 81 to 97% of Cd was associated with the cell walls. The largest amount and proportion (45-86%) of Cd was extracted with 2% acetic acid, and a concentration-dependent extraction was observed, both of which suggest that Cd-phosphate complexes were the major chemical form in E. pisciphila. A large distribution of phosphate and Cd on the mycelia surface was observed by scanning electron microscopy-energy dispersive spectrometer (SEM-EDS). The precipitates associated with the mycelia were observed to contain Cd by transmission electron microscopy-energy dispersive X-ray spectroscopy (TEM-EDX). Fourier transform infrared (FTIR) identified that hydroxyl, amine, carboxyl, and phosphate groups were responsible for binding Cd. We conclude that Cd associated with cell walls and integrated with phosphate might be responsible for the tolerance of E. pisciphila to Cd.
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纯培养物中深色有隔内生菌（DSE）对重金属的反应。
影响因子：
3.7
作者：
Ban Y;Tang M;Chen H;Xu Z;Zhang H;Yang Y
通讯作者：
Yang Y
影响因子：
5.7
作者：
Peixian Xu;Zhaolong Wang
通讯作者：
Peixian Xu;Zhaolong Wang
DOI：
--
发表时间：
2000
期刊：
Chinese Journal of Appplied Environmental Biology
影响因子：
--
作者：
Yang Zhimin;Zheng Shaojian;Hu Aitang
通讯作者：
Yang Zhimin;Zheng Shaojian;Hu Aitang
影响因子：
5.7
作者：
Q. Yao;Rui-Heng Yang;Liangkun Long;Honghui Zhu
通讯作者：
Q. Yao;Rui-Heng Yang;Liangkun Long;Honghui Zhu
DOI：
10.1016/s0304-4165(00)00090-8
发表时间：
2000-09-01
影响因子：
3
作者：
Bhanoori, M;Venkateswerlu, G
通讯作者：
Venkateswerlu, G