Cytotoxicity screening of single-walled carbon nanotubes: detection and removal of cytotoxic contaminants from carboxylated carbon nanotubes.
Cytotoxicity screening of single-walled carbon nanotubes: detection and removal of cytotoxic contaminants from carboxylated carbon nanotubes.
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DOI：
10.1021/mp2001439
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发表时间：
2011-08-01
影响因子：
4.9
通讯作者：
Draper RK
中科院分区：
文献类型：
--
作者：
Wang R;Mikoryak C;Li S;Bushdiecker D 2nd;Musselman IH;Pantano P;Draper RK
本研究比较了9种不同的单壁碳纳米管（SWNT）产品的细胞毒性培养的哺乳动物细胞的各种方法合成，并从供应商的横截面获得。一个标准的程序，涉及超声处理和离心缓冲牛血清白蛋白被开发分散在一个生物相容性的解决方案，以促进比较的所有单壁碳纳米管。然后评估SWNT对标准细胞系增殖能力的影响。在测试的九种不同的SWNT材料中，只有两种是显著有毒的，并且两者都是通过来自不同供应商的羧化而官能化的。这是出乎意料的，因为羧化使SWNT更具水溶性，这可能与更好的生物相容性相关。然而，额外的纯化工作表明，羧基化单壁碳纳米管制剂中的有毒物质可以通过过滤与单壁碳纳米管分离。含有毒性活性的滤液还含有丰富的小碳碎片，其具有无定形碳物质的拉曼特征，表明毒性和氧化碳碎片之间的相关性。去除与羧化单壁碳纳米管相关的有毒污染物在开发用于药理学应用的羧化单壁碳纳米管中是重要的。
This study compares the cytotoxicity to cultured mammalian cells of nine different single-walled carbon nanotube (SWNT) products synthesized by a variety of methods and obtained from a cross section of vendors. A standard procedure involving sonication and centrifugation in buffered bovine serum albumin was developed to disperse all the SWNTs in a biocompatible solution to facilitate comparisons. The effect of the SWNTs on the proliferative ability of a standard cell line was then assessed. Of the nine different SWNT materials tested, only two were significantly toxic, and both were functionalized by carboxylation from different vendors. This was unexpected because carboxylation makes SWNTs more water soluble, which would presumably correlate with better biocompatibility. However, additional purification work demonstrated that the toxic material in the carboxylated SWNT preparations could be separated from the SWNTs by filtration. The filtrate that contained the toxic activity also contained abundant small carbon fragments that had Raman signatures characteristic of amorphous carbon species, suggesting a correlation between toxicity and oxidized carbon fragments. The removal of a toxic contaminant associated with carboxylated SWNTs is important in the development of carboxylated SWNTs for pharmacological applications.
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影响因子：
17.1
作者：
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影响因子：
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