Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics.
Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics.
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DOI：
10.1155/2012/415697
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发表时间：
2012
影响因子：
2.6
通讯作者：
Strehlitz B
中科院分区：
文献类型：
--
作者：
Stoltenburg R;Nikolaus N;Strehlitz B
小的有机分子是使用SELEX技术(SELEX-通过指数浓缩的配体的系统进化)选择适体的具有挑战性的目标。它们通常不适合固定在固体表面，这是已知适配子选择方法中的常见程序。捕获-SELEX程序允许为溶质靶标选择DNA适配子。构建了一个特殊的SELEX文库，目的是将该文库固定在磁珠或其他表面上。为此，将对接序列加入到文库的随机区域中，使其能够与固定在磁珠上的互补寡核苷酸杂交。在适配子选择过程中，从珠子中释放文库中对靶标表现出高亲和力和与靶标匹配的二级结构的寡核苷酸，以与靶标结合。这些结合复合体的寡核苷酸被扩增、纯化并通过对接序列固定到磁珠上，作为下一轮选择的起点。基于这种捕获-SELEX程序，成功地选择了氨基糖苷类抗生素卡那霉素A作为小分子靶标的DNA适配子。
Small organic molecules are challenging targets for an aptamer selection using the SELEX technology (SELEX—Systematic Evolution of Ligans by EXponential enrichment). Often they are not suitable for immobilization on solid surfaces, which is a common procedure in known aptamer selection methods. The Capture-SELEX procedure allows the selection of DNA aptamers for solute targets. A special SELEX library was constructed with the aim to immobilize this library on magnetic beads or other surfaces. For this purpose a docking sequence was incorporated into the random region of the library enabling hybridization to a complementary oligo fixed on magnetic beads. Oligonucleotides of the library which exhibit high affinity to the target and a secondary structure fitting to the target are released from the beads for binding to the target during the aptamer selection process. The oligonucleotides of these binding complexes were amplified, purified, and immobilized via the docking sequence to the magnetic beads as the starting point of the following selection round. Based on this Capture-SELEX procedure, the successful DNA aptamer selection for the aminoglycoside antibiotic kanamycin A as a small molecule target is described.
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DOI：
10.3390/s100504541
发表时间：
2010
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Han K;Liang Z;Zhou N
通讯作者：
Zhou N
影响因子：
2.9
作者：
Allawi, HT;SantaLucia, J
通讯作者：
SantaLucia, J
影响因子：
4.8
作者：
Nutiu, R;Li, YF
通讯作者：
Li, YF
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
14.9
作者：
Chenna, R;Sugawara, H;Thompson, JD
通讯作者：
Thompson, JD