Association of different Candida species with catheter-related candidemia, and the potential antifungal treatments against their adhesion properties and biofilm-forming capabilities.
Association of different Candida species with catheter-related candidemia, and the potential antifungal treatments against their adhesion properties and biofilm-forming capabilities.
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不同念珠菌种类与导管相关念珠菌血症的关联，以及针对其粘附特性和生物膜形成能力的潜在抗真菌治疗
DOI：
10.1002/jcla.23738
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发表时间：
2021-04
影响因子：
2.7
通讯作者：
Hu K
中科院分区：
文献类型：
--
作者：
Zuo XS;Liu Y;Cai X;Zhan L;Hu K
比较从导管相关念珠菌血症患者体内获得的 27 株念珠菌分离株的粘附特性和生物膜形成能力，并评估抗真菌药物对不同念珠菌种类的抑制作用。研究了七种白色念珠菌、六种近平滑念珠菌、五种吉里蒙念珠菌、五种热带念珠菌和四种光滑念珠菌临床分离株。我们通过流式细胞仪方法量化了这些念珠菌物种的粘附，并通过 XTT 还原和结晶紫方法评估了生物膜的形成。确定了米卡芬净 (MF)、氟康唑 (FZ) 和 N-乙酰半胱氨酸 (NAC) 对不同念珠菌属的粘附和生物膜形成的作用。与白色念珠菌相比，非白色念珠菌被证明具有更强的粘附能力。不同念珠菌属物种的生物膜形成能力差异很大，生物膜形成的程度可能受到不同检测方法的影响。有趣的是，C. parapsilosis 表现出最高的生物膜形成能力，而 C. glabrata 表现出最低的总生物量和代谢活性。此外，还评估了MF、FZ和NAC对真菌粘附和生物膜形成的抑制活性，结果表明MF可以更显着地降低粘附能力和生物膜代谢（p < 0.05），并且其抗真菌活性以剂量依赖性方式升高。非白色念珠菌种，尤其是吉里蒙念珠菌、热带念珠菌和近平滑念珠菌，在导管相关念珠菌血症患者中表现出较高的粘附能力。然而，这些念珠菌物种具有不同的生物膜形成能力。 MF 对不同念珠菌属的粘附和生物膜形成具有更强的抑制作用。念珠菌粘附概况的表示。分离株具有弱、中、强和极强粘附模式的特征。均匀分布模式是指酵母细胞附着在等量的微球上；而异质分布模式表明多个微球结合到单个酵母细胞上。
To compare the adhesion properties and biofilm‐forming capabilities of 27 Candida isolates obtained from catheter‐related candidemia patients and to evaluate the inhibitory effects of antifungal agents on different Candida species. Seven C. albicans, six C. parapsilosis, five C. guilliermondii, five C. tropicalis, and four C. glabrata clinical isolates were investigated. We quantified the adherence of these Candida species by flow cytometric method and evaluated the formation of biofilms by XTT reduction and crystal violet methods. Actions of micafungin (MF), fluconazole (FZ), and N‐acetylcysteine (NAC) on the adhesion and biofilm formation of different Candida species were determined. Non‐albicans Candida species were demonstrated to have stronger adhesion abilities compared with C. albicans. The biofilm‐forming capabilities of different Candida species were varied considerably, and the degree of biofilm formation might be affected by different assay approaches. Interestingly, C. parapsilosis displayed the highest biofilm formation abilities, while C. glabrata exhibited the lowest total biomass and metabolic activity. Furthermore, the inhibitory activities of MF, FZ, and NAC on fungal adhesion and biofilm formation were evaluated, and the results indicated that MF could reduce the adhesion ability and biofilm metabolism more significantly (p < 0.05), and its antifungal activity was elevated in a dose‐dependent manner. Non‐albicans Candida species, especially C. guilliermondii, C. tropicalis, and C. parapsilosis, exhibited higher adhesion ability in catheter‐related candidemia patients. However, these Candida species had varied biofilm‐forming capabilities. MF tended to have stronger inhibitory effects against both adhesion and biofilm formation of different Candida species. Representation of Candida adhesion profiles. The isolates were characterized by weak, moderate, strong and very strong adhesion patterns. Homogeneous distribution pattern means that yeast cells are attached to an equal amount of microspheres; while heterogeneous distribution pattern indicates that multiple microspheres are bound to a single yeast cell.
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影响因子：
4.9
作者：
Pannanusorn, Srisuda;Fernandez, Victor;Romling, Ute
通讯作者：
Romling, Ute
影响因子：
5.2
作者：
Garnacho-Montero, Jose;Diaz-Martin, Ana;Lepe-Jimenez, Jose A.
通讯作者：
Lepe-Jimenez, Jose A.
影响因子：
3
作者：
Odds FC;Hanson MF;Davidson AD;Jacobsen MD;Wright P;Whyte JA;Gow NAR;Jones BL
通讯作者：
Jones BL
影响因子：
3.1
作者：
Mukherjee, PK;Chandra, J;Ghannoum, MA
通讯作者：
Ghannoum, MA
DOI：
10.1016/j.ijantimicag.2010.12.016
发表时间：
2011-04-01
影响因子：
10.8
作者：
Cateau, Estelle;Berjeaud, Jean-Marc;Imbert, Christine
通讯作者：
Imbert, Christine