Cell migration orchestrates migrasome formation by shaping retraction fibers.
Cell migration orchestrates migrasome formation by shaping retraction fibers.
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DOI：
10.1083/jcb.202109168
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发表时间：
2022-04-04
影响因子：
7.8
通讯作者：
Jiu, Yaming
中科院分区：
文献类型：
--
作者：
Fan, Changyuan;Shi, Xuemeng;Zhao, Kaikai;Wang, Linbo;Shi, Kun;Liu, Yan-Jun;Li, Hui;Ji, Baohua;Jiu, Yaming
范等人定量剖析了迁移模式（持续性和速度）对迁移体形成的决定性调控作用。迁移体是一种具有囊泡样形态的细胞器，介导迁移胞吐作用。他们的研究进一步加深了我们目前对细胞行为在迁移体研究中作用的理解。
迁移体是最近在迁移细胞的回缩纤维上发现的囊泡样结构，与细胞内容物转移、不需要物质的排出以及信息整合有关。然而，细胞迁移模式是否以及如何调控迁移体的形成尚不清楚。在此，我们报告称，与持续直线迁移的细胞相比，转向细胞中的迁移体显著减少。这一观察结果背后的主要原因是，随着细胞伸长，其后端变窄，从而在非持续迁移过程中产生的回缩纤维减少。除了迁移持续性，我们还发现迁移速度与迁移体形成呈正相关，这是由于回缩纤维的长度所致。为证实我们的假设，通过基因手段去除波形蛋白会降低细胞迁移速度和持续性，并导致迁移体数量减少。总之，我们的数据阐明了两种细胞迁移模式，即持续性和速度，通过调控回缩纤维在控制迁移体形成中所起的关键作用。
Fan et al. quantitatively dissect the decisive regulation of migration paradigm (persistence and velocity) to the formation of the migrasome, a cellular organelle with vesicle-like morphology that mediates migracytosis. Their work adds up our current understanding of the role of cell behavior in migrasome studies. Migrasomes are recently discovered vesicle-like structures on retraction fibers of migrating cells that have been linked with transfer of cellular contents, shedding of unwanted materials, and information integration. However, whether and how the cell migration paradigm regulates migrasome formation is not clear. Here, we report that there are significantly fewer migrasomes in turning cells compared with straight persistently migrating cells. The major insight underlying this observation is that as the cells elongate, their rear ends become narrower, subsequently resulting in fewer retraction fibers during impersistent migration. In addition to migration persistence, we reveal that migration speed positively corelates with migrasome formation, owing to the derived length of retraction fibers. Substantiating our hypothesis, genetically removing vimentin compromises cell migration speed and persistence and leads to fewer migrasomes. Together, our data explicate the critical roles of two cell migration patterns, persistence and speed, in the control of migrasome formation by regulating retraction fibers.
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影响因子：
8.8
作者：
Jiu, Yaming;Lehtimaki, Jaakko;Lappalainen, Pekka
通讯作者：
Lappalainen, Pekka
迁移体的形成是由微米级四跨膜蛋白宏结构域的组装介导的
影响因子：
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作者：
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作者：
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通讯作者：
Insall, Robert H.
影响因子：
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作者：
GAIL, MH;BOONE, CW
通讯作者：
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