Investigating the influence of moxibustion on colonic mucosal barrier in rats with dextran sulfate sodium-induced ulcerative colitis
Investigating the influence of moxibustion on colonic mucosal barrier in rats with dextran sulfate sodium-induced ulcerative colitis
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艾灸对右旋糖酐硫酸钠诱导溃疡性结肠炎大鼠结肠黏膜屏障的影响
DOI：
10.1007/s11726-022-1286-8
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发表时间：
2022-02
影响因子：
0.5
通讯作者：
Ma Zhe
中科院分区：
文献类型：
--
作者：
Shen Ya;Cui Yunhua;Shi Zheng;Wu Huangan;Wang Zhaoqin;Wu Luyi;Lu Yuan;Huang Yan;Liu Yanan;Long Junyi;Lin Yaying;Ma Zhe
目的观察艾灸对葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）大鼠结肠黏膜屏障的影响。造模组采用4%DSS连续灌胃7 d制备实验性UC模型。随机选取2只造模大鼠和2只正常大鼠进行模型鉴定。待UC模型成功后，将剩余18只造模大鼠随机分为模型组、模型+隔药饼灸组、模型+温和灸组，每组6只;将剩余的正常大鼠随机分为三组：正常组，正常+隔药饼灸组，正常+温和灸组，每组6只。隔药饼灸或温和灸干预7 d后，采用HE染色技术，光镜下观察结肠组织的病理变化;采用免疫印迹和/或免疫组化技术检测细胞内封闭蛋白（Occludin）、紧密连接蛋白（Claudin）、连接粘附分子1（JAM 1）、粘蛋白2（MUC 2）、结果与正常组相比，模型组大鼠结肠组织损伤严重，病理评分明显增加，Occludin、Claudin、JAM 1、MUC 2、TGF-β1蛋白表达水平明显降低正常+隔药饼灸组与正常+温和灸组结肠组织病理学评分、Occludin、Claudin、JAM 1、MUC 2、TGF-β1蛋白表达水平差异无统计学意义（P> 0. 05）。与模型组相比，模型+隔药饼灸组和模型+温和灸组结肠组织修复，溃疡愈合，病理评分明显降低，JAM 1、MUC 2、TGF-β1蛋白表达水平明显升高（P<0.05）;模型+温和灸组结肠组织中Occludin蛋白表达水平升高结论隔药饼灸和温和灸对正常大鼠结肠组织病理学及肠黏膜屏障相关蛋白表达均无影响;隔药饼灸和温和灸均可上调UC大鼠结肠组织中JAM 1、MUC 2和TGF-β1的蛋白表达水平。温和灸可上调Occludin蛋白表达。这可能是艾灸减轻UC结肠黏膜炎症的机制之一。
ObjectiveTo observe the effect of moxibustion on the colonic mucosal barrier of rats with ulcerative colitis (UC) induced by dextran sulfate sodium (DSS).MethodsForty male Sprague-Dawley rats were randomly divided into a normal group and a modeling group, with 20 rats in each group. Rats in the modeling group were subjected to preparing experimental UC models by drinking 4% DSS for seven consecutive days. Two modeled rats and two normal rats were randomly selected for model identification. After the success of UC model was confirmed, the remaining 18 modeled rats were randomly divided into three groups, a model group, a model + herbal cake-partitioned moxibustion group, and a model + mild moxibustion group, with six rats in each group; the remaining normal rats were randomly divided into three groups, a normal group, a normal + herbal cake-partitioned moxibustion group, and a normal + mild moxibustion group, with six rats in each group. After 7 d of intervention with the herbal cake-partitioned moxibustion or the mild moxibustion, hematoxylin-eosin (HE) staining technique was used to observe the pathological changes of colon tissue under a light microscope; Western blotting and/or immunohistochemical techniques were used to detect the protein expression levels of Occludin, Claudin, junction adhesion molecular 1 (JAM1), mucin 2 (MUC2), and transforming growth factor beta1 (TGF-β1) in rat colon tissue.ResultsCompared with the normal group, the colon tissue was severely damaged, the pathological score was significantly increased, and the protein expression levels of Occludin, Claudin, JAM1, MUC2, and TGF-β1 were significantly decreased in the model group (P<0.01); while there were no significant differences in the colonic histopathological score, protein expression levels of Occludin, Claudin, JAM1, MUC2, and TGF-β1 in the normal + herbal cake-partitioned moxibustion group and the normal + mild moxibustion group (P>0.05). Compared with the model group, the model + herbal cake-partitioned moxibustion group and the model + mild moxibustion group showed repaired colon tissue, ulcer healing, significantly reduced pathological score, and significantly increased protein expression levels of JAM1, MUC2, and TGF-β1 (P<0.05); the Occludin protein expression level in the colon tissue of the model + mild moxibustion group was increased (P<0.01).ConclusionNeither herbal cake-partitioned moxibustion nor mild moxibustion influences the colonic histopathology and intestinal mucosal barrier-related protein expression in the normal rats; both herbal cake-partitioned moxibustion and mild moxibustion can up-regulate the protein expression levels of JAM1, MUC2, and TGF-β1 in the colon tissue of UC rats. Mild moxibustion can up-regulate Occludin protein expression. This may be a mechanism of moxibustion in reducing colonic mucosa inflammation in UC.
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影响因子：
1.1
作者：
Lin, Ya-Ying;Zhao, Ji-Meng;Wu, Huan-Gan
通讯作者：
Wu, Huan-Gan
影响因子：
3.9
作者：
Vancamelbeke M;Vermeire S
通讯作者：
Vermeire S
影响因子：
4.3
作者：
Ma, Tie-Ming;Xu, Na;Yan, Hong-Chi
通讯作者：
Yan, Hong-Chi
影响因子：
3.7
作者：
Wenzel UA;Magnusson MK;Rydström A;Jonstrand C;Hengst J;Johansson ME;Velcich A;Öhman L;Strid H;Sjövall H;Hansson GC;Wick MJ
通讯作者：
Wick MJ
隔药灸对 DSS 溃疡性结肠炎大鼠结肠 miRNA 表达谱的影响
DOI：
10.1155/2017/1767301
发表时间：
2017
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Huang Y;Ma Z;Cui YH;Dong HS;Zhao JM;Dou CZ;Liu HR;Li J;Wu HG
通讯作者：
Wu HG