Maturation of Oral Microbiota in Children with or without Dental Caries.
Maturation of Oral Microbiota in Children with or without Dental Caries.
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DOI：
10.1371/journal.pone.0128534
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Johansson I
中科院分区：
文献类型：
--
作者：
Lif Holgerson P;Öhman C;Rönnlund A;Johansson I
这项纵向研究的目的是评估3个月至3岁儿童的口腔微生物群，并确定3岁时龋齿存在的相关性。在3个月（n = 207）和3岁（n = 155）时对儿童的口腔生物膜和唾液进行采样，并在3岁时对龋齿进行评分。通过培养总乳酸杆菌和变形链球菌、PCR检测变形链球菌和远缘链球菌、454焦磷酸测序和HOMIM（Human Oral Microbe Identification Microarray）微阵列检测超过300种/种类型的口腔微生物群。物种丰富度和类群多样性显着增加，从3个月到3年。所有3个月大的婴儿中存在的三个细菌属在3岁时仍然存在，而其他三个属在这个年龄时已经消失。在3岁时也观察到大量的新类群。3个月龄时的微生物群，除了乳酸杆菌，与以后年龄的龋齿发展无关。相反，3岁儿童口腔生物膜中的几个分类群与龋齿的存在或不存在有关。与3岁儿童龋齿相关的主要种属/菌群类型属于放线菌属、变形菌属、聚集菌属和链球菌属，而影响龋齿不存在的种属/菌群类型属于放线菌属、Bergeyella、弯曲杆菌属、颗粒菌属、Kingella、Leptotrichia和链球菌属。因此，在生命的最初几年，物种丰富度和类群多样性在口中显着增加。除了更普遍的乳酸杆菌定植外，3个月龄时的总体微生物群组成与以后年龄的龋齿发展无关。3岁儿童口腔生物膜中的几个分类群可能与龋齿的存在或不存在有关。
The aim of this longitudinal study was to evaluate the oral microbiota in children from age 3 months to 3 years, and to determine the association of the presence of caries at 3 years of age. Oral biofilms and saliva were sampled from children at 3 months (n = 207) and 3 years (n = 155) of age, and dental caries was scored at 3 years of age. Oral microbiota was assessed by culturing of total lactobacilli and mutans streptococci, PCR detection of Streptococcus mutans and Streptococcus sobrinus, 454 pyrosequencing and HOMIM (Human Oral Microbe Identification Microarray) microarray detection of more then 300 species/ phylotypes. Species richness and taxa diversity significantly increased from 3 months to 3 years. Three bacterial genera, present in all the 3-month-old infants, persisted at 3 years of age, whereas three other genera had disappeared by this age. A large number of new taxa were also observed in the 3-year-olds. The microbiota at 3 months of age, except for lactobacilli, was unrelated to caries development at a later age. In contrast, several taxa in the oral biofilms of the 3-year-olds were linked with the presence or absence of caries. The main species/phylotypes associated with caries in 3-year-olds belonged to the Actinobaculum, Atopobium, Aggregatibacter, and Streptococcus genera, whereas those influencing the absence of caries belonged to the Actinomyces, Bergeyella, Campylobacter, Granulicatella, Kingella, Leptotrichia, and Streptococcus genera. Thus, during the first years of life, species richness and taxa diversity in the mouth increase significantly. Besides the more prevalent colonization of lactobacilli, the composition of the overall microbiota at 3 months of age was unrelated to caries development at a later age. Several taxa within the oral biofilms of the 3-year-olds could be linked to the presence or absence of caries.
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影响因子：
7.3
作者：
Arrieta MC;Stiemsma LT;Amenyogbe N;Brown EM;Finlay B
通讯作者：
Finlay B
影响因子：
7.6
作者：
Könönen, E;Kanervo, A;Jousimies-Somer, H
通讯作者：
Jousimies-Somer, H
影响因子：
3.6
作者：
Ling, Zongxin;Kong, Jianming;Xiang, Charlie
通讯作者：
Xiang, Charlie
影响因子：
2.7
作者：
Crielaard W;Zaura E;Schuller AA;Huse SM;Montijn RC;Keijser BJ
通讯作者：
Keijser BJ
影响因子：
3.1
作者：
Lundqvist A;Johansson I;Wennberg A;Hultdin J;Högberg U;Hamberg K;Sandström H
通讯作者：
Sandström H