Bis-enoxacin blocks rat alveolar bone resorption from experimental periodontitis.
Bis-enoxacin blocks rat alveolar bone resorption from experimental periodontitis.
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DOI：
10.1371/journal.pone.0092119
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kesavalu L
中科院分区：
文献类型：
--
作者：
Rivera MF;Chukkapalli SS;Velsko IM;Lee JY;Bhattacharyya I;Dolce C;Toro EJ;Holliday LS;Kesavalu L
牙周病是多因素的，由牙龈下的多种微生物病原体引起，包括牙龈卟啉单胞菌、牙齿密螺旋体和连翘Tannerella。慢性牙周感染导致炎症、结缔组织破坏、牙周韧带破坏和牙槽骨吸收，最终导致牙齿脱落。依诺沙星和依诺沙星的双膦酸衍生物（双依诺沙星）抑制破骨细胞的形成和骨吸收，也含有抗生素特性。我们的研究表明，依诺沙星和/或双依诺沙星可能有助于减少牙槽骨吸收和可能的细菌定植。用109个由牙龈假单胞菌、齿牙假单胞菌和连翘假单胞菌组成的多微生物接种物感染大鼠，每隔一周灌洗一次，持续12周。多微生物感染6周后，每天皮下注射依诺沙星（5mg /kg/天）、双依诺沙星（5,25 mg/kg/天）、阿仑膦酸钠（1,10 mg/kg/天）或多西环素（5mg /天）。在安乐死后评估牙周病参数，包括细菌定植/感染、免疫反应、炎症、牙槽骨吸收和全身扩散。在多微生物感染期间，所有三种牙周病原体都在大鼠口腔定植。多微生物感染导致牙槽骨吸收总量增加、骨内缺损和牙龈炎症。与阿仑膦酸钠或强力霉素相比，双依诺星治疗显著降低了牙槽骨吸收。组织学检查显示，双依诺沙星和依诺沙星治疗可减轻牙龈炎症，减少连接上皮的根尖迁移。这些数据支持双依诺沙星和依诺沙星可能对治疗牙周病有用的假设。
Periodontal diseases are multifactorial, caused by polymicrobial subgingival pathogens, including Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. Chronic periodontal infection results in inflammation, destruction of connective tissues, periodontal ligament, and alveolar bone resorption, and ultimately tooth loss. Enoxacin and a bisphosphonate derivative of enoxacin (bis-enoxacin) inhibit osteoclast formation and bone resorption and also contain antibiotic properties. Our study proposes that enoxacin and/or bis-enoxacin may be useful in reducing alveolar bone resorption and possibly bacterial colonization. Rats were infected with 109 cells of polymicrobial inoculum consisting of P. gingivalis, T. denticola, and T. forsythia, as an oral lavage every other week for twelve weeks. Daily subcutaneous injections of enoxacin (5 mg/kg/day), bis-enoxacin (5, 25 mg/kg/day), alendronate (1, 10 mg/kg/day), or doxycycline (5 mg/day) were administered after 6 weeks of polymicrobial infection. Periodontal disease parameters, including bacterial colonization/infection, immune response, inflammation, alveolar bone resorption, and systemic spread, were assessed post-euthanasia. All three periodontal pathogens colonized the rat oral cavity during polymicrobial infection. Polymicrobial infection induced an increase in total alveolar bone resorption, intrabony defects, and gingival inflammation. Treatment with bis-enoxacin significantly decreased alveolar bone resorption more effectively than either alendronate or doxycycline. Histologic examination revealed that treatment with bis-enoxacin and enoxacin reduced gingival inflammation and decreased apical migration of junctional epithelium. These data support the hypothesis that bis-enoxacin and enoxacin may be useful for the treatment of periodontal disease.
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影响因子：
4.8
作者：
Lee, BS;Gluck, SL;Holliday, LS
通讯作者：
Holliday, LS
影响因子：
4.3
作者：
Karimbux, NY;Ramamurthy, NS;Nishimura, I
通讯作者：
Nishimura, I
DOI：
10.1002/hed.23235
发表时间：
2013-12-01
影响因子：
2.9
作者：
Lee, Jang-Jaer;Cheng, Shih-Jung;Kok, Sang-Heng
通讯作者：
Kok, Sang-Heng
影响因子：
7.3
作者：
Ostrov DA;Magis AT;Wronski TJ;Chan EK;Toro EJ;Donatelli RE;Sajek K;Haroun IN;Nagib MI;Piedrahita A;Harris A;Holliday LS
通讯作者：
Holliday LS
影响因子：
3.1
作者：
Kesavalu, Lakshmyya;Sathishkumar, Sabapathi;Ebersole, Jeffrey L.
通讯作者：
Ebersole, Jeffrey L.