The joining of blunt DNA ends to 3'-protruding single strands in Escherichia coli.
The joining of blunt DNA ends to 3'-protruding single strands in Escherichia coli.
复制标题
大肠杆菌中钝 DNA 末端与 3 突出单链的连接。
DOI：
10.1093/nar/26.7.1749
复制
发表时间：
1998
影响因子：
14.9
通讯作者：
Morgan,W
中科院分区：
文献类型：
--
作者：
King,J;Fairley,C;Morgan,W
In eukaryotic and prokaryotic organisms DNA double-strand breaks with non-complementary ends can be joined by mechanisms of illegitimate recombination. We examined the joining of 3′-protruding single strand (PSS) ends, which do not have recessed 3′ hydroxyls that can allow for fill-in DNA synthesis, to blunt ends. End-joining was examined by electro-transformingEscherichia colistrains with linearized plasmid DNA, sequencing the resulting junctions, and determining the transformation frequencies. Three differentE.colistrains were examined: MC1061, which has no known recombination or DNA repair defects, HB101 (recA-) and SURE (recB−recJ−). No striking differences were found in either the spectrum of products observed or the efficiency of end-joining between these strains. As in vertebrate systems, the majority of the products were overlaps between directly repeated DNA sequences. 3′-PSS are frequently preserved in vertebrate systems, but they were not preserved in our experiments unless the transforming DNA was pretreated with a DNA polymerase.
登录
查看更多内容
细菌和人类细胞中非互补 DNA 双链断裂重新连接。
影响因子：
14.9
作者：
King,JS;Valcarcel,ER;Rufer,JT;Phillips,JW;Morgan,WF
通讯作者：
Morgan,WF
影响因子：
4.8
作者：
Connelly, JC;deLeau, ES;Leach, DRF
通讯作者：
Leach, DRF
影响因子：
14.9
作者：
RALEIGH, EA;MURRAY, NE;HANAHAN, D
通讯作者：
HANAHAN, D
由特定类型的限制性内切酶诱导的双链断裂产生的突变谱。
影响因子：
2.7
作者：
Winegar,RA;Lutze,LH;Rufer,JT;Morgan,WF
通讯作者：
Morgan,WF
影响因子：
64.5
作者：
THODE, S;SCHAFER, A;VIELMETTER, W
通讯作者：
VIELMETTER, W