Identification and characterization of a Chinese isolate of Cryptosporidium serpentis from dairy cattle
Identification and characterization of a Chinese isolate of Cryptosporidium serpentis from dairy cattle
复制标题
奶牛蛇形隐孢子虫中国分离株的鉴定和表征
DOI：
10.1007/s00436-012-3024-5
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发表时间：
2012-07
影响因子：
2
通讯作者：
Qiu, Huiling
中科院分区：
文献类型：
--
作者：
Chen, Fu;Qiu, Huiling
隐孢子虫蛇是一种首先在蛇身上观察到的原生动物，在蜥蜴和其他爬行动物身上也发现了。然而，很少有从人类和其他动物中分离出来的蛇形球菌特征的报道。本研究从小牛分离的蛇形螺旋体进行形态学、宿主特异性和小亚基核糖体RNA （SSU rRNA）和热休克蛋白70 （HSP 70）基因序列的表征。卵囊尺寸为6.32 × 5.18 μm，长/宽形状指数为1.22。交叉传播研究表明，分离物在BALB/c小鼠胃中具有传染性，但在新西兰大白兔和白来角鸡胃中无传染性。SSU rRNA和hsp70基因的序列分析和系统发育分析表明，该分离物与C. serentis同源，属于C. serentis单系类群。这项研究首次描述了从奶牛中分离出的蛇隐孢子虫的特征，可能有助于更好地了解蛇隐孢子虫和调查牛隐孢子虫的流行情况。
Cryptosporidium serpentis, a protozoan observed first in snakes, has also been found in lizards and other reptiles. However, there are few reports of the characteristics of C. serpentis isolated from humans and other animals. The present study was undertaken to characterize a C. serpentis isolate from a calf in terms of morphology, host specificity, and small-subunit ribosomal RNA (SSU rRNA) and heat shock protein 70 (HSP 70) gene sequences. Oocysts of the isolate measured 6.32 × 5.18 μm, and they had a length/width shape index of 1.22. A cross-transmission study demonstrated that the isolate was infectious in the stomach of BALB/c mice, but not in New Zealand white rabbits or white leghorn chickens. Sequence and phylogenetic analysis of the SSU rRNA and HSP 70 gene revealed that the isolate was identical to C. serpentis, and it was classified in a monophyletic group of C. serpentis. This study is the first description of the characteristics of a C. serpentis isolate from dairy cattle and may contribute to a better understanding of C. serpentis and investigations of the prevalence of Cryptosporidium in cattle.
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DOI：
--
发表时间：
2007
期刊：
Molekuliarnaia biologiia
影响因子：
--
作者：
M. Azami;D. D. Moghaddam-D.;R. Salehi;M. Salehi
通讯作者：
M. Azami;D. D. Moghaddam-D.;R. Salehi;M. Salehi
影响因子：
2.6
作者：
Fayer, Ronald;Santin, Monica;Trout, James M.
通讯作者：
Trout, James M.
影响因子：
2
作者：
R. Zhou;Guoqing Li;S. Xiao;Yanxun Xia;Y. Guo
通讯作者：
R. Zhou;Guoqing Li;S. Xiao;Yanxun Xia;Y. Guo
影响因子：
2.6
作者：
Saki Nagano;M. Matsubayashi;T. Kita;T. Narushima;I. Kimata;M. Iseki;Tomoya Hajiri;H. Tani;K. Sasai;E. Baba
通讯作者：
Saki Nagano;M. Matsubayashi;T. Kita;T. Narushima;I. Kimata;M. Iseki;Tomoya Hajiri;H. Tani;K. Sasai;E. Baba
影响因子：
2.6
作者：
Feltus, Dawn C.;Giddings, Catherine W.;McEvoy, John M.
通讯作者：
McEvoy, John M.