Antiproliferative and apoptosis inducing effect of essential oil extracted from Cyrtomium fortumei (J.) Smith leaves
Antiproliferative and apoptosis inducing effect of essential oil extracted from Cyrtomium fortumei (J.) Smith leaves
复制标题
贯众叶精油的抗增殖和细胞凋亡诱导作用
DOI：
10.1007/s00044-014-1244-1
复制
发表时间：
2014-09
影响因子：
2.6
通讯作者：
Yang, Song
中科院分区：
文献类型：
--
作者：
Zhao, Qi;Zhao, Hongju;Xue, Wei;Yang, Song
贯众Cyrtomium fortumei（J.）史密斯是中国特有种，已被证明是一种重要的中草药。然而，C. fortumei（J.）史密斯的离开仍然不清楚。本研究采用超临界CO2萃取法（SFE-CO2）从该植物中提取EO，并采用MTT法研究其对肿瘤细胞MGC-803、MCF-7、BGC-823、Bcap-37、A375和A549的体外作用。经GC-MS分析鉴定了26个化合物，其中EO对MGC-803、Bcap-37和A549等肿瘤细胞具有明显的抗肿瘤活性（IC 50值为0.15 ~ 0.24 mg/mL），并对其主要成分进行了活性研究。随后的荧光染色和流式细胞仪分析表明，EO可诱导MGC-803、Bcap-37和A549细胞凋亡，在0.15 mg/mL的EO作用48 h后，MGC-803细胞的凋亡率达到26.44%。MGC-803细胞中的半胱天冬酶3活性也被测定，当细胞用油处理时，与对照相比，半胱天冬酶3酶的活性增加。本研究表明，从C. fortumei（J.）Smith能抑制人癌细胞的生长，并能诱导癌细胞凋亡。
Cyrtomium fortumei(J.) Smith is an endemic species in China, which has been proved to be an important Chinese herbal medicine. However, chemical composition and bioactivity of essential oil (EO) ofC. fortumei(J.) Smith leaves remain unclear. In present study, we isolated EO from the plant by supercritical carbon dioxide extraction assay (SFE-CO2), and investigated on cancer cells MGC-803, MCF-7, BGC-823, Bcap-37, A375, and A549 in vitro by MTT assay. 26 compounds were identified by GC-MS analysis, and the EO showed significant antitumor activities against MGC-803, Bcap-37, and A549 cancer cell lines (IC50values ranging from 0.15 to 0.24 mg/mL), and the activities of its main component were also studied. Subsequent fluorescence staining and flow cytometry analysis indicated that the EO could induce apoptosis in MGC-803, Bcap-37, and A549 cell lines, and the apoptosis ratios reached 26.44 % after 48 h of treatment at 0.15 mg/mL in MGC-803 cells. Caspase 3 activity in MGC-803 cells was also determined when the cells treated with the oil, and the activity of caspase 3 enzyme was increased compared to the control. This study suggests that the EO isolated fromC. fortumei(J.) Smith could inhibit the growth of human carcinoma cells, and it could induce apoptosis of cancer cells.
登录
查看更多内容
DOI：
10.1016/s2221-1691(13)60076-3
发表时间：
2013-05-01
影响因子：
1.7
作者：
Al Nomaani, Rahma Said Salim;Hossain, Mohammad Amzad;Al-Sabahi, Jamal Nasser
通讯作者：
Al-Sabahi, Jamal Nasser
香椿成分对人癌细胞的抗增殖活性和诱导细胞凋亡机制
影响因子：
5.8
作者：
Yang S;Zhao Q;Xiang H;Liu M;Zhang Q;Xue W;Song B;Yang S
通讯作者：
Yang S
贯众根茎成分的发现及其抗肿瘤活性
影响因子：
--
作者：
Yang S;Liu M;Liang N;Zhao Q;Zhang Y;Xue W;Yang S
通讯作者：
Yang S
影响因子：
0.7
作者：
El-Alfy TS;Ezzat SM;Hegazy AK;Amer AM;Kamel GM
通讯作者：
Kamel GM
影响因子：
0.7
作者：
Choi SY
通讯作者：
Choi SY