β1/2 or M2/3 Receptors Are Required for Different Gastrointestinal Motility Responses Induced by Acupuncture at Heterotopic or Homotopic Acupoints.
β1/2 or M2/3 Receptors Are Required for Different Gastrointestinal Motility Responses Induced by Acupuncture at Heterotopic or Homotopic Acupoints.
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DOI：
10.1371/journal.pone.0168200
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhu B
中科院分区：
文献类型：
--
作者：
Gao X;Zhao Y;Su Y;Liu K;Yu X;Cui C;Yang Z;Shi H;Jing X;Zhu B
已知针刺同位穴位或异位穴位可以抑制或促进胃肠蠕动，具体取决于穴位位置。然而，很少有人致力于研究特定受体（例如肾上腺素能和毒蕈碱乙酰胆碱受体）在介导针刺异位和同位穴位效应中的作用。不同的肾上腺素能受体亚型或胆碱能受体亚型主要在肠道的不同部位表达，导致针刺异位或同位穴位对胃肠动力的影响存在差异。在这里，我们通过同时记录远端结肠和胃或空肠的管腔内压力并检查β1/2受体敲除小鼠和M2/3受体敲除小鼠的粪便酚红排泄，研究了β1/β2受体和M2/M3受体在针刺异位穴位ST37和同位穴位ST25调节胃肠运动中的作用。我们发现M2/3受体的敲除显着抑制了ST37针灸引起的胃动力、空肠动力和结肠动力的增强。此外，敲除β1/2受体显着减弱了ST25针灸诱导的胃动力和空肠动力抑制，而没有显着改变针灸在ST25诱导的结肠动力增强。 ST37 针刺显着加速了 β1/2 受体敲除小鼠及其野生型同窝小鼠的胃肠道转变。然而，相对于野生型同窝小鼠，M2/3 受体敲除小鼠中这种胃肠道转变的加速明显减弱。 ST25 针刺可显着增加 β1/2 受体敲除小鼠的胃肠道转变，并显着降低 M2/3 受体敲除小鼠的胃肠道转变，而不会改变野生型同窝小鼠的胃肠道转变。我们的研究表明，M2/3受体是与异位穴位针刺增强的整个胃肠道转变相关的胃肠道运动所必需的，而β1/2受体是同位穴位针刺抑制的相同胃肠道运动过程所必需的。因此，我们的研究结果揭示了针灸治疗胃肠动力功能障碍疾病的重要生物学机制。
Acupuncture at homotopic acupoints or heterotopic acupoints is known to either inhibit or facilitate gastrointestinal motility, depending on the acupoint location. However, little effort has been made to investigate the roles of specific receptors (such as adrenergic and muscarinic acetylcholine receptors) in mediating the effects of acupuncture at heterotopic and homotopic acupoints. Different adrenergic receptor subtypes or cholinergic receptor subtypes are predominantly expressed in various sections of the gut, resulting in variations between the effects of acupuncture at heterotopic or homotopic acupoints on gastrointestinal motility. Here, we investigated the role of β1/β2 receptors and M2/M3 receptors in gastrointestinal motility regulated by acupuncture at ST37, a heterotopic acupoint, and ST25, a homotopic acupoint, by simultaneously recording intraluminal pressures in the distal colon and stomach or jejunum and examining fecal phenol red excretion in β1/2 receptor-knockout mice and M2/3 receptor-knockout mice. We found that knockout of the M2/3 receptor significantly inhibited ST37 acupuncture-induced enhancement of gastric motility, jejunal motility, and colonic motility. Additionally, knocking out of the β1/2 receptor significantly diminished the ST25 acupuncture-induced inhibition of gastric motility and jejunal motility without significantly altering the enhancement of colonic motility induced by acupuncture at ST25. Acupuncture at ST37 significantly accelerated gastrointestinal transition in β1/2 receptor-knockout mice and their wild-type littermates. However, this acceleration of gastrointestinal transition was markedly diminished in M2/3 receptor-knockout mice relative to their wild-type littermates. Acupuncture at ST25 significantly increased gastrointestinal transition in β1/2 receptor-knockout mice and significantly decreased gastrointestinal transition in M2/3 receptor-knockout mice without altering gastrointestinal transition in wild-type littermates of either. Our study revealed that M2/3 receptors are required for the gastrointestinal motility associated with whole gastrointestinal transition enhanced by acupuncture at heterotopic acupoints, whereas β1/2 receptors are required for the same gastrointestinal motility processes inhibited by acupuncture at homotopic acupoints. Therefore, our findings reveal important biological mechanisms underlying acupuncture treatment of disorders involving gastrointestinal motility dysfunction.
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DOI：
10.1152/ajpgi.00401.2013
发表时间：
2014-07-15
影响因子：
4.5
作者：
Lomasney, Kevin W.;Cryan, John F.;Hyland, Niall P.
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TERUI, N
DOI：
10.1073/pnas.97.17.9579
发表时间：
2000-08-15
影响因子：
11.1
作者：
Matsui, M;Motomura, D;Taketo, MM
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