Hypoxic ischaemic hypothermia promotes neuronal differentiation and inhibits glial differentiation from newly generated cells in the SGZ of the neonatal rat brain
Hypoxic ischaemic hypothermia promotes neuronal differentiation and inhibits glial differentiation from newly generated cells in the SGZ of the neonatal rat brain
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缺氧缺血低温促进新生大鼠大脑 SGZ 神经元分化并抑制新生细胞的胶质细胞分化
DOI：
10.1016/j.neulet.2012.06.054
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发表时间：
2012-08
影响因子：
2.5
通讯作者：
Zhou, Wen-Hao
中科院分区：
文献类型：
--
作者：
Xiong, Man;Ma, Si-Min;Shao, Xiao-Mei;Yang, Yi;Zhou, Wen-Hao
低温是治疗成人和新生儿脑缺氧缺血性损伤的潜在疗法。发育中的大鼠大脑缺氧缺血（HI）后低温的神经保护作用机制仍不清楚。在这项研究中，7天大的大鼠接受了左颈动脉结扎，然后给予8%的氧气2小时。 HI后立即将这些大鼠分为低温组（直肠温度32-33°C，24小时）和常温组（36-37°C，24小时）。所有大鼠在 HI 后 4-6 天腹腔注射 50mg/kg/天 5-溴脱氧尿苷 (BrdU)，并在 1 或 2 周处死。我们发现，与常温组相比，HI 后 7 天和 14 天，低温组的梗塞体积显着减少，颗粒下区 (SGZ) 也检测到神经元丢失。与常温组相比，低温组BrdU免疫阳性细胞明显减少。 HI 后 1 周和 2 周，低温并没有改变同侧 SGZ 中巢蛋白标记的细胞数量。通过 BrdU 与胶质纤维酸性蛋白 (GFAP)、O4 或神经元核 (NeuN) 的双重免疫标记来评估新生成细胞的分化。 HI后2周和6周，低温组与常温组相比，BrdU+–GFAP+或BrdU+–O4+占总BrdU+染色的比率显着下降，但BrdU+–NeuN+占总BrdU+染色的比率显着增加。这些结果表明，亚低温后观察到的神经元损失减少可能与HI后SGZ神经元分化增强和神经胶质分化减少有关。这些观察结果对于围产期脑损伤后的临床低温治疗值得注意。
Hypothermia is a potential therapy for cerebral hypoxic ischaemic injury in adults and neonates. The mechanism of the neuroprotective effects of hypothermia after hypoxia–ischaemia (HI) in the developing rat brain remains unclear. In this research, 7-day-old rats underwent left carotid artery ligation followed by the administration of 8% oxygen for 2h. These rats were divided into hypothermic (rectal temperature, 32–33°C for 24h) and normothermic (36–37°C for 24h) groups immediately after HI. All rats were given 50mg/kg/day 5-bromodeoxyuridine (BrdU) intraperitoneally at 4–6 days and sacrificed at 1 or 2 weeks after HI. We found a significant decrease in infarct volume and the neuron loss were also detected in the subgranular zone (SGZ) in the hypothermic group at 7 and 14 days after HI compared with the normothermic group. BrdU immunopositive cells were reduced greatly in the hypothermic group compared with the normothermic group. Hypothermia did not change the number of nestin-labelled cells in the ipsilateral SGZ at 1 and 2 weeks after HI. The differentiation of newly generated cells was assessed by double immunolabelling of BrdU with glial fibrillary acidic protein (GFAP), O4 or Neuronal Nuclei (NeuN). The ratio of BrdU+–GFAP+or BrdU+–O4+to total BrdU+staining decreased dramatically, but the ratio of BrdU+–NeuN+to total BrdU+staining increased significantly in the hypothermic group compared to the normothermic group at 2 and 6 weeks after HI. These results suggest that the reduction in neuron loss observed after mild hypothermia may be associated with enhanced neuronal differentiation and decreased glial differentiation in the SGZ after HI. These observations are noteworthy for clinical hypothermia therapy following cerebral HI injury during the perinatal period.
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影响因子：
2.9
作者：
Hayashi, T;Iwai, M;Abe, K
通讯作者：
Abe, K
影响因子：
6.3
作者：
G. Silasi;F. Colbourne
通讯作者：
G. Silasi;F. Colbourne
影响因子：
1.2
作者：
J. Fujita
通讯作者：
J. Fujita
影响因子：
2.5
作者：
Xie, Yan-Chun;Li, Cheng-Yan;Ye, Fei
通讯作者：
Ye, Fei
影响因子：
2.5
作者：
SHANKARAN, S;WOLDT, E;NANDYAL, R
通讯作者：
NANDYAL, R