Oxidative stress induces coordinated remodeling of RNA-enzyme interactions.
Oxidative stress induces coordinated remodeling of RNA-enzyme interactions.
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DOI：
10.1016/j.isci.2021.102753
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发表时间：
2021-07-23
期刊：
影响因子：
5.8
通讯作者：
Gerber AP
中科院分区：
文献类型：
--
作者：
Matia-González AM;Jabre I;Laing EE;Gerber AP
RNA结合蛋白（RBP）是关键的转录后调节因子，在胁迫适应过程中发挥重要作用。最近的蛋白质组范围的调查已经发现了大量的新的和“非常规”的RBP，如代谢酶，但很少有人知道的重组的RNA结合蛋白质组（RBPome）和RNA酶的相互作用，以响应细胞应激。在这里，我们应用RNA相互作用组捕获监测mRBPome在酵母酿酒酵母中的轻度氧化应激后的动力学。在257种显著改变RNA结合的蛋白质中，我们观察到RNA结合酶的协调重塑-特别是中心碳代谢-补充了已知的代谢反应。此外，我们认识到酶-RNA相互作用的旁系同源特异性改变的倾向。我们的研究结果表明，协调的RNA-酶的相互作用和中间代谢之间的串扰，以维持氧化应激后的生理和分子平衡，也许通过专业化的旁系同源进化过程中。氧化应激诱导257种蛋白质在多聚腺苷酸化RNA上的重排RNA-酶相互作用和代谢的协调反应酵母RNA结合酶具有特异性三种不同质谱分析工具的整合分子网络;微生物代谢;蛋白质组学
RNA-binding proteins (RBPs) are key post-transcriptional regulators that play a substantial role during stress adaptation. Recent proteome-wide surveys have uncovered a large number of new and “unconventional” RBPs such as metabolic enzymes, yet little is known about the reconfiguration of the RNA-binding proteome (RBPome) and RNA-enzyme interactions in response to cellular stress. Here, we applied RNA-interactome capture to monitor the dynamics of the mRBPome upon mild oxidative stress in the yeast Saccharomyces cerevisiae. Among the 257 proteins that significantly changed RNA associations, we observed the coordinated remodeling of RNA-binding enzymes — particularly of the central carbon metabolism — that complemented known metabolic responses. Furthermore, we recognized the propensity for paralogous specific alterations of enzyme-RNA interactions. Our results suggest coordinated cross talk between RNA-enzyme interactions and intermediary metabolism to maintain the physiological and molecular balance upon oxidative stress, perhaps through specialization of paralogous during evolution. Oxidative stress induces the rearrangement of 257 proteins on polyadenylated RNAs Coordinated response of RNA-enzyme interactions and metabolism Yeast RNA-binding enzymes are paralog specific Integration of three different mass spectrometry analysis tools Molecular network; Microbial metabolism; Proteomics
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影响因子：
4.5
作者：
Kershaw CJ;Costello JL;Castelli LM;Talavera D;Rowe W;Sims PF;Ashe MP;Hubbard SJ;Pavitt GD;Grant CM
通讯作者：
Grant CM
影响因子：
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Higami Y
影响因子：
16.8
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Gerber AP
影响因子：
16.6
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通讯作者：
Hentze MW
影响因子：
30.8
作者：
Hieronymus, H;Silver, PA
通讯作者：
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