Adult mouse basal forebrain harbors two distinct cholinergic populations defined by their electrophysiology.
Adult mouse basal forebrain harbors two distinct cholinergic populations defined by their electrophysiology.
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DOI：
10.3389/fnbeh.2012.00021
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发表时间：
2012
影响因子：
3
通讯作者：
Zaborszky L
中科院分区：
文献类型：
--
作者：
Unal CT;Golowasch JP;Zaborszky L
我们进行了全细胞记录从基底前脑（BF）胆碱能神经元的转基因小鼠表达增强型绿色荧光蛋白（eGFP）的胆碱乙酰转移酶启动子的控制下。BF胆碱能神经元可分为两个电生理学上可识别的亚型：早放电神经元和晚放电神经元。早期放电神经元（约70%）更易兴奋，表现出突出的尖峰频率适应，更容易受到去极化阻滞，这是一种以初始动作电位后神经元完全沉默为特征的现象。晚放电神经元（约30%），虽然兴奋性较低，但可以维持低频的紧张性放电。在电压钳分析中，我们已经表明，早期放电神经元具有更高密度的低电压激活（LVA）钙电流。这两个胆碱能细胞群可能参与不同的功能：早期放电组更适合于与注意力相关的皮层乙酰胆碱释放的阶段性变化，而晚期放电神经元可以通过维持紧张性乙酰胆碱水平来支持一般唤醒。
We performed whole-cell recordings from basal forebrain (BF) cholinergic neurons in transgenic mice expressing enhanced green fluorescent protein (eGFP) under the control of the choline acetyltransferase promoter. BF cholinergic neurons can be differentiated into two electrophysiologically identifiable subtypes: early and late firing neurons. Early firing neurons (∼70%) are more excitable, show prominent spike frequency adaptation and are more susceptible to depolarization blockade, a phenomenon characterized by complete silencing of the neuron following initial action potentials. Late firing neurons (∼30%), albeit being less excitable, could maintain a tonic discharge at low frequencies. In voltage clamp analysis, we have shown that early firing neurons have a higher density of low voltage activated (LVA) calcium currents. These two cholinergic cell populations might be involved in distinct functions: the early firing group being more suitable for phasic changes in cortical acetylcholine release associated with attention while the late firing neurons could support general arousal by maintaining tonic acetylcholine levels.
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