Early MR diffusion and relaxation changes in the parahippocampal gyrus precede the onset of spontaneous seizures in an animal model of chronic limbic epilepsy.
Early MR diffusion and relaxation changes in the parahippocampal gyrus precede the onset of spontaneous seizures in an animal model of chronic limbic epilepsy.
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DOI：
10.1016/j.expneurol.2010.03.031
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发表时间：
2010-07
影响因子：
5.3
通讯作者：
Mareci, Thomas H.
中科院分区：
文献类型：
--
作者：
Parekh, Mansi B.;Carney, Paul R.;Sepulveda, Hector;Norman, Wendy;King, Michael;Mareci, Thomas H.
关键词：
在颞叶癫痫（TLE）患者和动物模型中经常观察到边缘区域的结构变化。然而，在演变为癫痫的过程中，大脑结构的变化在很大程度上仍然未知。因此，本研究的目的是通过定量弥散张量成像（DTI）和T2弛豫仪在慢性TLE动物模型中确定体内癫痫发生潜伏期实验性癫痫持续状态（SE）后边缘结构的时间变化。一对50微米的电极被植入12只成年雄性大鼠的腹侧海马体。采用电刺激诱导11只大鼠自持性SE。三只大鼠作为年龄匹配的对照组。在11.1特斯拉、电极植入前和植入后以及se后3、5、7、10、20、40和60天进行体内扩散张量和T2磁共振成像（MRI）以评估结构变化。自发性癫痫发作由连续时间锁定视频监控识别。在体内成像后，对固定的、切除的大脑进行17.6特斯拉的磁共振成像。随后，将组织学分析与MRI结果相关联。SE后，8/11的损伤大鼠出现自发性癫痫发作。这8只大鼠在体内观察到海马旁回（对侧）和膜（双侧）的早期T2、弥散性和各向异性变化。在切除的大脑中，在齿状回观察到双侧各向异性增加，对应于苔状纤维发芽，通过Timm染色确定。使用T2弛豫仪和DTI，仅在发生自发性边缘癫痫发作的大鼠中观察到特异性的短暂和长期结构变化。
Structural changes in limbic regions are often observed in individuals with temporal lobe epilepsy (TLE) and in animal models. However, the brain structural changes during the evolution into epilepsy remain largely unknown. Therefore, the purpose of this study is to define the temporal changes in limbic structures after experimental status epilepticus (SE) during the latency period of epileptogenesis in vivo, with quantitative diffusion tensor imaging (DTI) and T2 relaxometry in an animal model of chronic TLE. A pair of fifty micron electrodes was implanted into the ventral hippocampus in twelve male adult rats. Self-sustaining SE was induced with electrical stimulation in eleven rats. Three rats served as age-matched controls. In vivo diffusion tensor and T2 magnetic resonance imaging (MRI) was performed at 11.1 Tesla, pre- and post-implantation of electrodes and 3, 5, 7, 10, 20, 40 and 60 days post-SE to assess structural changes. Spontaneous seizures were identified with continuous time-locked video-monitoring. Following imaging in vivo, fixed, excised brains were MR imaged at 17.6 Tesla. Subsequently, histological analysis was correlated with MRI results. Following SE, 8/11 injured rats developed spontaneous seizures. Unique to these 8 rats, early T2, diffusivity and anisotropy changes were observed in vivo within the parahippocampal gyrus (contralateral) and fimbria (bilateral). In excised brains, bilateral increase in anisotropy was observed in the dentate gyrus, corresponding to mossy fiber sprouting as determined by Timm staining. Using T2 relaxometry and DTI, specific transient and long-term structural changes were observed only in rats that developed spontaneous limbic seizures.
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影响因子：
11.2
作者：
BERTRAM, EH;LOTHMAN, EW;LENN, NJ
通讯作者：
LENN, NJ
影响因子：
9.9
作者：
Bernasconi, N;Bernasconi, A;Reutens, DC
通讯作者：
Reutens, DC
影响因子：
5.6
作者：
Bertram, EH
通讯作者：
Bertram, EH
影响因子：
5.6
作者：
HAUSER, WA;ANNEGERS, JF;KURLAND, LT
通讯作者：
KURLAND, LT
影响因子：
5.6
作者：
Concha, Luis;Beaulieu, Christian;Gross, Donald W.
通讯作者：
Gross, Donald W.