Strain dependent gene expression and neurochemical levels in the brain of zebrafish: focus on a few alcohol related targets.
Strain dependent gene expression and neurochemical levels in the brain of zebrafish: focus on a few alcohol related targets.
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DOI：
10.1016/j.physbeh.2012.01.017
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发表时间：
2012-12-05
影响因子：
2.9
通讯作者：
Gerlai, R.
中科院分区：
文献类型：
--
作者：
Pan, Y.;Chatterjee, D.;Gerlai, R.
斑马鱼在行为遗传学中越来越受欢迎，因为它可以进行大规模的突变和药物筛选，从而有助于发现复杂性状的机制。成年斑马鱼行为的品系差异已经有报道，这可能对神经行为遗传学有重要意义。例如，我们发现AB和SF菌株对急性和慢性酒精暴露的行为反应不同。在目前的研究中，我们进一步表征这些菌株使用半定量RT-PCR来测量10个选定的基因的表达和HPLC来测量9种神经化学物质的水平。我们选择的靶基因和神经化学物质的基础上，他们在酒精和其他药物滥用相关的机制的潜在参与。我们定量了编码D1-R、D2 a-R、D4 a-R多巴胺受体、GABAA-R、GABAB-R1、GAD 1、MAO、NMDA-R（NR 2D亚基）、5 HT-R1 bd和SLC 6 a4 a的基因的表达。我们发现，与AB相比，编码D1多巴胺受体的基因在SF中过表达，而编码GABAB 1受体和溶质家族载体蛋白6（SLC 6）4a的基因在SF中低表达。我们还发现，与AB相比，SF中所有（多巴胺、DOPAC、5-羟色胺、GABA、谷氨酸、甘氨酸、天冬氨酸、牛磺酸）神经化学物质的水平均降低，但有一种（5 HIAA）神经化学物质的水平降低。这些结果，结合先前确定的AB和SF菌株之间的行为差异，证明了斑马鱼菌株表征的重要性。他们现在还允许制定工作假设的可能机制的差异影响急性和慢性酒精治疗这两个斑马鱼品系。
The zebrafish is becoming increasingly popular in behavior genetics because it may allow one to conduct large scale mutation and drug screens facilitating the discovery of mechanisms of complex traits. Strain differences in adult zebrafish behavior have already been reported, which may have important implications in neurobehavioral genetics. For example, we have found the AB and SF strains to differ in their behavioral responses to both acute and chronic alcohol exposure. In the current study, we further characterize these strains using semi-quantitative RT-PCR to measure the expression of ten selected genes and HPLC to measure the levels of nine neurochemicals. We chose the target genes and neurochemicals based upon their potential involvement in alcohol and other drugs of abuse related mechanisms. We quantified the expression of the genes encoding D1-R, D2a-R, D4a-R dopamine receptors, GABAA-R, GABAB-R1, GAD1, MAO, NMDA-R (NR2D subunit), 5HT-R1bd and SLC6 a4a. We found the gene encoding D1 dopamine receptor over-expressed and the genes encoding GABAB1 receptor and solute family carrier protein 6 (SLC6) 4a under-expressed in SF compared to AB. We also found the level of all (dopamine, DOPAC, Serotonin, GABA, Glutamate, Glycine, Aspartate, Taurine) but one (5HIAA) neurochemicals tested decreased in SF as compared to AB. These results, combined with previously identified behavioral differences between the AB and SF strains, demonstrate the importance of strain characterization in zebrafish. They now also allow formulation of working hypotheses about possible mechanisms underlying the differential effects of acute and chronic alcohol treatment on these two zebrafish strains.
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影响因子：
2.7
作者：
Egan, Rupert J.;Bergner, Carisa L.;Hart, Peter C.;Cachat, Jonathan M.;Canavello, Peter R.;Elegante, Marco F.;Elkhayat, Salem I.;Bartels, Brett K.;Tien, Anna K.;Tien, David H.;Mohnot, Sopan;Beeson, Esther;Glasgow, Eric;Amri, Hakima;Zukowska, Zofia;Kalueff, Allan V.
通讯作者：
Kalueff, Allan V.
影响因子：
5
作者：
Lesch, KP
通讯作者：
Lesch, KP
影响因子：
15.9
作者：
Gerlai, R
通讯作者：
Gerlai, R
影响因子：
2.3
作者：
Maccioni, Paola;Colombo, Giancarlo
通讯作者：
Colombo, Giancarlo
影响因子：
2.5
作者：
Chastain, Garvin
通讯作者：
Chastain, Garvin