Spatial mapping of tracheal ciliary beat frequency using real time phase-resolved Doppler spectrally encoded interferometric microscopy.
Spatial mapping of tracheal ciliary beat frequency using real time phase-resolved Doppler spectrally encoded interferometric microscopy.
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DOI：
10.1021/acsphotonics.9b01235
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发表时间：
2020-01-15
期刊：
影响因子：
7
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
He Y;Jing JC;Qu Y;Wong BJ;Chen Z
上呼吸道中的纤毛运动是身体将外来颗粒输送出呼吸系统以维持适当呼吸功能的主要机制。纤毛搏动频率（CBF）通常随着疾病的发作以及其他条件（例如温度的变化或对药物施用的响应）而中断。目前睫状体运动的成像依赖于显微镜和高速相机，其不能容易地适应于体内成像。M模式光学相干断层扫描（OCT）成像能够可视化纤毛活动，但视野有限。我们报告的频谱编码干涉显微镜（SEIM）系统的发展，使用相位分辨多普勒（PRD）算法来测量和映射的纤毛跳动频率内的正面区域。这种新型的高速，高分辨率的系统允许可视化的时间和空间的纤毛运动模式，以及传播的异时波。本实验观察了不同温度条件下家兔气管CBF的变化，频率范围为9 ~ 13 Hz，并测定了利多卡因和沙丁胺醇对CBF的影响。这项研究是在体内研究和翻译的成像空间CBF的临床垫脚石。
Ciliary motion in the upper airway is the primary mechanism by which the body transports foreign particulates out of the respiratory system in order to maintain proper respiratory function. The ciliary beating frequency (CBF) is often disrupted with the onset of disease as well as other conditions, such as changes in temperature or in response to drug administration. Current imaging of ciliary motion relies on microscopy and high-speed cameras, which cannot be easily adapted to in-vivo imaging. M-mode optical coherence tomography (OCT) imaging is capable of visualization of ciliary activity, but the field of view is limited. We report on the development of a spectrally encoded interferometric microscopy (SEIM) system using a phase-resolved Doppler (PRD) algorithm to measure and map the ciliary beating frequency within an en face region. This novel high speed, high resolution system allows for visualization of both temporal and spatial ciliary motion patterns as well as propagation of metachronal wave. Rabbit tracheal CBF ranging from 9 to 13 Hz has been observed under different temperature conditions, and the effects of using lidocaine and albuterol have also been measured. This study is the stepping stone to in-vivo studies and the translation of imaging spatial CBF to clinics.
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