Enzymes in the glutamate-glutamine cycle in the anterior cingulate cortex in postmortem brain of subjects with autism.
Enzymes in the glutamate-glutamine cycle in the anterior cingulate cortex in postmortem brain of subjects with autism.
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DOI：
10.1186/2040-2392-4-6
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发表时间：
2013-03-26
期刊：
影响因子：
6.2
通讯作者：
Mori N
中科院分区：
文献类型：
--
作者：
Shimmura C;Suzuki K;Iwata Y;Tsuchiya KJ;Ohno K;Matsuzaki H;Iwata K;Kameno Y;Takahashi T;Wakuda T;Nakamura K;Hashimoto K;Mori N
越来越多的证据表明，谷氨酸能系统的功能障碍可能是自闭症病理生理学的基础。前扣带皮层（ACC）与自闭症和多巴胺能神经传递有关。我们假设ACC中谷氨酸-谷氨酰胺循环的改变可能在自闭症的病理生理学中起作用。我们进行蛋白质印迹分析的蛋白质表达水平的酶在谷氨酸-谷氨酰胺循环，包括谷氨酰胺合成酶，肾型转氨酶，肝型转氨酶，谷氨酸脱氢酶1和2，在ACC的个体与自闭症（n = 7）和对照组（n = 13）的死后大脑。我们发现，与对照组相比，自闭症受试者ACC中肾型转氨酶的蛋白质水平显著降低，而其他酶的蛋白质水平则没有变化。结果表明，减少表达的肾型转氨酶可能占假定的改变，在自闭症的ACC中的多巴胺能神经传递。
Accumulating evidence suggests that dysfunction in the glutamatergic system may underlie the pathophysiology of autism. The anterior cingulate cortex (ACC) has been implicated in autism as well as in glutamatergic neurotransmission. We hypothesized that alterations in the glutamate-glutamine cycle in the ACC might play a role in the pathophysiology of autism. We performed Western blot analyses for the protein expression levels of enzymes in the glutamate-glutamine cycle, including glutamine synthetase, kidney-type glutaminase, liver-type glutaminase, and glutamate dehydrogenases 1 and 2, in the ACC of postmortem brain of individuals with autism (n = 7) and control subjects (n = 13). We found that the protein levels of kidney-type glutaminase, but not those of the other enzymes measured, in the ACC were significantly lower in subjects with autism than in controls. The results suggest that reduced expression of kidney-type glutaminase may account for putative alterations in glutamatergic neurotransmission in the ACC in autism.
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影响因子：
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