In vivo dynamic 3D imaging of oocytes and embryos in the mouse oviduct.
In vivo dynamic 3D imaging of oocytes and embryos in the mouse oviduct.
复制标题
卵母细胞和胚胎的体内动态3D成像。
DOI：
10.1016/j.celrep.2021.109382
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发表时间：
2021-07-13
期刊：
影响因子：
8.8
通讯作者：
Larina IV
中科院分区：
文献类型：
--
作者：
Wang S;Larina IV
发育生物学家一直依赖成像来阐明动态细胞事件。然而，哺乳动物受精和胚胎发生等过程通常无法直接成像。因此，输卵管（输卵管）如何促进配子和植入前胚胎的运输仍然没有答案。在这里，我们提出了一种结合活体窗口和光学相干断层扫描的动态，体积，在体内成像的卵母细胞和胚胎，因为它们是通过小鼠输卵管运输。我们观察到卵母细胞和胚胎的位置依赖性盘旋，振荡和长距离双向运动，这表明驱动运输的调节机制，并质疑该领域的既定观点。这种体内成像方法可以与各种遗传和药理学操作相结合，用于活体功能分析，从而有可能在其天然状态下研究生殖生理学。Wang和Larina利用光学相干断层扫描和活体显微镜对卵母细胞和胚胎通过小鼠输卵管运输时的体内体积成像进行了研究。这项研究揭示了卵母细胞和胚胎的复杂动力学，表明纤毛和输卵管收缩在驱动运输中的调节作用。
Developmental biologists have always relied on imaging to shed light on dynamic cellular events. However, processes such as mammalian fertilization and embryogenesis are generally inaccessible for direct imaging. In consequence, how the oviduct (fallopian tube) facilitates the transport of gametes and preimplantation embryos continues to be unanswered. Here we present a combination of intravital window and optical coherence tomography for dynamic, volumetric, in vivo imaging of oocytes and embryos as they are transported through the mouse oviduct. We observed location-dependent circling, oscillating, and long-distance bi-directional movements of oocytes and embryos that suggest regulatory mechanisms driving transport and question established views in the field. This in vivo imaging approach can be combined with a variety of genetic and pharmacological manipulations for live functional analysis, bringing the potential to investigate reproductive physiology in its native state. Wang and Larina present in vivo volumetric imaging of oocytes and embryos as they are transported through the mouse oviduct with optical coherence tomography and an intravital microscopy. The study reveals complex dynamics of oocytes and embryos that suggest a regulatory role of cilia and oviductal contractions in driving the transport.
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DOI：
10.1159/000301391
发表时间：
1976-01-01
期刊：
GYNECOLOGIC INVESTIGATION
影响因子：
--
作者：
HALBERT, SA;TAM, PY;BLANDAU, RJ
通讯作者：
BLANDAU, RJ
影响因子：
4.4
作者：
Georgiou, A. Stephen;Snijders, Ambrosius P. L.;Fazeli, Alireza
通讯作者：
Fazeli, Alireza
影响因子：
3.4
作者：
Burton, Jason C.;Wang, Shang;Larina, Irina V.
通讯作者：
Larina, Irina V.
DOI：
10.1530/jrf.0.0560515
发表时间：
1979-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BATTALIA, DE;YANAGIMACHI, R
通讯作者：
YANAGIMACHI, R
影响因子：
3.6
作者：
HALBERT, SA;BECKER, DR;SZAL, SE
通讯作者：
SZAL, SE