Tr-milRNA1 Contributes to Lignocellulase Secretion under Heat Stress by Regulating the Lectin-Type Cargo Receptor Gene Trvip36 in Trichoderma guizhouence NJAU 4742.
Tr-milRNA1 Contributes to Lignocellulase Secretion under Heat Stress by Regulating the Lectin-Type Cargo Receptor Gene Trvip36 in Trichoderma guizhouence NJAU 4742.
复制标题
Tr-milRNA 1通过调节凝集素型Cargo受体基因Trvip 36促进热胁迫下木霉NJAU 4742分泌纤维素酶
DOI：
10.3390/jof7120997
复制
发表时间：
2021-11-23
期刊：
影响因子：
--
通讯作者：
Shen Q
中科院分区：
文献类型：
--
作者：
Li T;Liu J;Wang Q;Liu Y;Li T;Liu D;Shen Q
背景：MicroRNA通过调控相应的靶基因在多种生物学过程中发挥重要作用。然而，真菌microRNA-like RNA（milRNA）的生物学功能和调控机制仍知之甚少。研究方法：本研究结合深度测序和生物信息学分析，在28 °C和37 °C条件下，以稻草为唯一碳源，对贵州木霉NJAU 4742的milRNA及其靶基因进行了分离和鉴定。结果如下：关键milRNA TGA1_S04_31828（Tr-milRNA 1）在热胁迫（37 °C）下高度表达并适应性地调节木质纤维素酶分泌。过表达Tr-milRNA 1（OE-Tr-milRNA 1）不影响营养生长，但显著提高了热胁迫下木质纤维素的利用率。基于生物信息学分析和qPCR验证，Tr-milRNA 1的靶标被鉴定为Trvip 36，一种凝集素型货物受体。随着温度从28 °C升高到37 °C，Tr-milRNA 1和Trvip 36在SSF条件下的表达量呈现逐渐增加的趋势。此外，Trvip 36在Tr-milRNA 1过表达株（OE-Tr-milRNA 1）中的表达受到显著抑制。与野生型相比，缺失Trvip 36（Δ Trvip 36）可显著提高热胁迫下木质纤维素酶在内质网中的滞留量，从而促进木质纤维素酶的分泌。结论：来自NJAU 4742的Tr-milRNA 1通过调节相应靶基因Trvip 36的表达来提高热胁迫下木质纤维素的利用。这些发现为探索丝状真菌分泌木质纤维素酶的机制提供了新的途径。
Background: MicroRNA plays an important role in multifarious biological processes by regulating their corresponding target genes. However, the biological function and regulatory mechanism of fungal microRNA-like RNAs (milRNAs) remain poorly understood. Methods: In this study, combined with deep sequencing and bioinformatics analysis, milRNAs and their targets from Trichoderma guizhouence NJAU 4742 were isolated and identified under solid-state fermentation (SSF) by using rice straw as the sole carbon source at 28 °C and 37 °C, respectively. Results: A critical milRNA, TGA1_S04_31828 (Tr-milRNA1), was highly expressed under heat stress (37 °C) and adaptively regulated lignocellulase secretion. Overexpression of Tr-milRNA1 (OE-Tr-milRNA1) did not affect vegetative growth, but significantly increased lignocellulose utilization under heat stress. Based on the bioinformatics analysis and qPCR validation, a target of Tr-milRNA1 was identified as Trvip36, a lectin-type cargo receptor. The expression of Tr-milRNA1 and Trvip36 showed a divergent trend under SSF when the temperature was increased from 28 °C to 37 °C. In addition, the expression of Trvip36 was suppressed significantly in Tr-milRNA1 overexpression strain (OE-Tr-milRNA1). Compared with the wild type, deletion of Trvip36 (ΔTrvip36) significantly improved the secretion of lignocellulases by reducing the retention of lignocellulases in the ER under heat stress. Conclusions: Tr-milRNA1 from NJAU 4742 improved lignocellulose utilization under heat stress by regulating the expression of the corresponding target gene Trvip36. These findings might open avenues for exploring the mechanism of lignocellulase secretion in filamentous fungi.
登录
查看更多内容
DOI：
10.1007/s00018-010-0471-y
发表时间：
2010-11
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Li L;Chang SS;Liu Y
通讯作者：
Liu Y
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
DOI：
10.1038/nrg3355
发表时间：
2013-02
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.8
作者：
Bo, XC;Wang, SQ
通讯作者：
Wang, SQ